{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:27:35Z","timestamp":1767007655807,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD30 transmembrane receptor, a member of the tumor necrosis factor receptor family, is expressed in different lymphomas. Brentuximab vedotin (BV), a CD30 monoclonal antibody (Ab)\u2010drug conjugate, is effective in CD30\u2010positive lymphomas. However, the response to BV is not always correlated to CD30 expression detected by immunohistochemistry (IHC). The objectives of this study were to standardize and evaluate CD30 intensity by flow cytometry (FCM) in non\u2010Hodgkin's lymphomas. Twelve centers analyzed 161 cases on standardized cytometers using normalized median fluorescence intensity (nMFI30) of three different Abs, of which one clone can recognize the same epitope as BV. FCM distinguished four groups of cases: negative group (<jats:italic>n<\/jats:italic>\u00a0=\u00a0110) which showed no expression with the three clones; high positive group (<jats:italic>n<\/jats:italic>\u00a0=\u00a013) which gave nMFI30\u2009&gt;\u20095% with all tested clones; dim positive group (<jats:italic>n<\/jats:italic>\u00a0=\u00a017) which showed nMFI30\u2009&gt;\u20091% with all tested clones and &lt;5% for at least one; discordant group (<jats:italic>n<\/jats:italic>\u00a0=\u00a021) with positive and negative expression of the different clones. In consistency with the literature, CD30 was positive in all anaplastic large cell lymphomas, in some diffuse large B\u2010cell lymphomas (DLBCL), and in other rare lymphomas. FCM results were concordant with those of IHC in 77% of cases. Discrepancies could be explained by clones\u2010related differences, microenvironment, or intracytoplasmic staining. Interestingly, FCM was more sensitive than IHC in 11% of cases, especially in DLBCL. Multicenter standardized FCM of specific CD30 could improve case detection and extend the treatment of BV to various CD30\u2010positive lymphomas.<\/jats:p>","DOI":"10.1002\/cyto.b.21940","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T20:02:45Z","timestamp":1598558565000},"page":"488-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Multicentric MFI30 study: Standardization of flow cytometry analysis of CD30 expression in non\u2010Hodgkin lymphoma"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8124-3925","authenticated-orcid":false,"given":"Agathe","family":"Debliquis","sequence":"first","affiliation":[{"name":"Laboratoire d'H\u00e9matologie Groupe Hospitalier de la r\u00e9gion Mulhouse Sud Alsace  Mulhouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucile","family":"Baseggio","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie Cellulaire Groupement Hospitalier Sud\/Hospices Civils de Lyon  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Bouyer","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique Center Hospitalier Universitaire de Poitiers  Poitiers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Guy","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie biologique Center Hospitalier Universitaire de Dijon  Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine","family":"Garnache\u2010Ottou","sequence":"additional","affiliation":[{"name":"Laboratoire H\u00e9matologie, UMR1098, EFS BFC Universit\u00e9 de Franche Comt\u00e9  Besan\u00e7on France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franck","family":"Genevieve","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, Center Hospitalier Universitaire d'Angers F\u00e9d\u00e9ration Hospitalo\u2010Universitaire \u201cGrand Ouest Against Leukemia\u201d (FHU GOAL)  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Mayeur\u2010Rousse","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie Center Hospitalier Universitaire de Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remi","family":"Letestu","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique H\u00f4pital Avicenne HUPSSD, AP\u2010HP  Bobigny France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0601-3948","authenticated-orcid":false,"given":"Nicolas","family":"Chapuis","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique Hopital Cochin APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e9ronique","family":"Harrivel","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie CHU Amiens\u2010Picardie  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hind","family":"Bennani","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie Hopital Foch  Suresnes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastien","family":"Lachot","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologie Center Hospitalier Universitaire de Tours  Tours France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Loosveld","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie Center Hospitalier Universitaire de Marseille, CNRS, INSERM, CIML, Aix Marseille Universit\u00e9  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne","family":"Nicolino\u2010Brunet","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie et Biologie Vasculaire du Pr Fran\u00e7oise Dignat George Center Hospitalier Universitaire La Conception  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u00ebl","family":"P\u00e9r\u00e8s","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie IUCT\u2010Oncopole, CHU de Toulouse  Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikael","family":"Roussel","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Center Hospitalier Universitaire de Rennes  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Veyrat\u2010Masson","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, H\u00f4pital Estaing Center Hospitalier Universitaire de Clermont\u2010Ferrand  Clermont\u2010Ferrand France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Christine","family":"Jacob","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunologie Center Hospitalier Universitaire de Grenoble\u2010Alpes  La Tronche France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Drenou","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie Groupe Hospitalier de la r\u00e9gion Mulhouse Sud Alsace  Mulhouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.1.2491755"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2016.1256481"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2012.081935"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1708984"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.029256"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21403"},{"key":"e_1_2_7_8_1","first-page":"1167","article-title":"Quantification of CD38 expression in B\u2010cell chronic lymphocytic leukemia (B\u2010CLL): A comparison between antibody binding capacity (ABC) and relative median fluorescence (RMF)","volume":"45","author":"Dignum H. M.","year":"2004","journal-title":"Leukemia & Lymphoma"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.5858\/2004-128-1004-AOFCAI"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.1.1.bloodjournal8511"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-01-0039"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.5858\/133.5.756"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.49.7.539"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.12835"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/112.2.241"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32984-2"},{"volume-title":"ISO\/CEI 17043\u20102010: Conformity assessment\u2014General requirements for proficiency testing","year":"2010","author":"International Organization for Standardization (ISO)","key":"e_1_2_7_17_1"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.122"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13647"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-2069"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1990.tb00709.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31266-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.38.0402"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22404"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23844"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.4.848.848"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1309\/123P-CE6V-WYAK-BB1F"},{"volume-title":"WHO classification of tumours of haematopoietic and lymphoid tissues","year":"2017","author":"Swerdlow S. H.","key":"e_1_2_7_28_1"},{"key":"e_1_2_7_29_1","first-page":"1123","article-title":"Classical Hodgkin's disease. Clinical impact of the immunophenotype","volume":"151","author":"Wasielewski R.","year":"1997","journal-title":"The American Journal of Pathology"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000347192"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2010.080846"},{"key":"e_1_2_7_32_1","first-page":"855","article-title":"The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors","volume":"9","author":"Yeste\u2010Velasco M.","year":"2019","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1002965"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23106"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11903"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2009.00725.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2012.12.006"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21940","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21940","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T07:07:06Z","timestamp":1693292826000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,17]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1002\/cyto.b.21940"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21940","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"type":"print","value":"1552-4949"},{"type":"electronic","value":"1552-4957"}],"subject":[],"published":{"date-parts":[[2020,8,17]]},"assertion":[{"value":"2020-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}