{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:31:12Z","timestamp":1787315472942,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The proliferation marker Ki\u201067 is widely used within the field of diagnostic histopathology as a prognostic marker for solid cancers. However, Ki\u201067 is hardly used for prognostic and diagnostic purposes in flow cytometric analyses of hematologic neoplasms. In the present study, we investigated to what extent the proliferative activity, as determined by Ki\u201067 expression, is disturbed in myeloproliferative neoplasms (MPN), myelodysplastic syndrome (MDS), and MDS\/MPN diseases. Bone marrow aspirates from 74 patients suffering from MPN, MDS, or MDS\/MPN, and aspirates from 50 non\u2010malignant cases were analyzed by flow cytometry for Ki\u201067 expression in the erythro\u2010, myelo\u2010, and monopoiesis. Ki\u201067 expression was used to investigate the proliferative activity during the various maturation steps within these hematopoietic cell lineages. In the MPN patient cohort, the proliferative activity of all cell lineages is significantly higher during almost all maturation stages compared to those of the benign control cohort. In the MDS and MDS\/MPN cohort, a significantly lower proliferative activity is observed in the early maturation stages. In the MDS\/MPN patient cohort, increased proliferative activity is seen in the later stages of the maturation. MDS and MDS\/MPN display a distinct pattern in the proliferating fraction of maturing hematopoietic cells. This could become of added value in order to classify these malignancies based on their biological background and behavior, as well as in gaining a better understanding into the pathobiology of these malignancies.<\/jats:p>","DOI":"10.1002\/cyto.b.21946","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T18:50:55Z","timestamp":1598640655000},"page":"322-330","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Proliferative activity is disturbed in myeloproliferative neoplasms (\n                    <scp>MPN<\/scp>\n                    ), myelodysplastic syndrome (\n                    <scp>MDS<\/scp>\n                    ), and\n                    <scp>MDS<\/scp>\n                    \/\n                    <scp>MPN<\/scp>\n                    diseases. Differences between\n                    <scp>MDS<\/scp>\n                    and\n                    <scp>MDS<\/scp>\n                    \/\n                    <scp>MPN<\/scp>"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4694-8484","authenticated-orcid":false,"given":"Stefan G. C.","family":"Mestrum","sequence":"first","affiliation":[{"name":"Department of Molecular Cell Biology, GROW\u2010School for Oncology and Developmental Biology Maastricht University Medical Center  Maastricht The Netherlands"},{"name":"Department of Clinical Chemistry and Hematology Zuyderland Medical Center  Sittard\u2010Geleen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert C. J.","family":"de Wit","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory (CDL) Maastricht University Medical Center  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roosmarie J. M.","family":"Drent","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Hematology Zuyderland Medical Center  Sittard\u2010Geleen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton H. N.","family":"Hopman","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, GROW\u2010School for Oncology and Developmental Biology Maastricht University Medical Center  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans C. S.","family":"Ramaekers","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, GROW\u2010School for Oncology and Developmental Biology Maastricht University Medical Center  Maastricht The Netherlands"},{"name":"Nordic\u2010MUbio  Susteren The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Math P. G.","family":"Leers","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Hematology Zuyderland Medical Center  Sittard\u2010Geleen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.110"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-018-0142-y"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11899-015-0269-y"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18610"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21065"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.133.4.1710"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112317108"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21125"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197233"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2012.45"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044321"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-696005"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-032490"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23564"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-460170"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1386.031"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24396"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.89"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-04-736421"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-10-844670"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11188"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.92.4.531"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-500272"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-018-0843-7"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.13079"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000018"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21946","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21946","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21946","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T21:24:23Z","timestamp":1693603463000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21946"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,28]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1002\/cyto.b.21946"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21946","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,28]]},"assertion":[{"value":"2020-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}