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Here, we investigated the in vitro hypersensitivity of lymphocytes from Fanconi anemia (FA) patients, to cytotoxic drugs using CDA. Peripheral blood mononuclear cells (PBMC) as well as cell lines derived from FA patients were treated with two DNA interstrand crosslinking (ICL) agents, mitomycin C and cyclophosphamide. Our data indicate that the CDA detects hypersensitivity of cells from FA patients to mitomycin C. Further, cell lines derived from FA\u2010patients were also hypersensitive to mitomycin C as well as cyclophosphamide, when assayed by the CDA. This study suggests that the CDA is a useful alternative for the diagnosis of FA patients' hypersensitivity to ICL agents.<\/jats:p>","DOI":"10.1002\/cyto.b.21950","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T22:52:19Z","timestamp":1598655139000},"page":"370-376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Use of the cell division assay to diagnose <scp>Fanconi<\/scp> anemia patients' hypersensitivity to mitomycin <scp>C<\/scp>"],"prefix":"10.1002","volume":"100","author":[{"given":"Ola","family":"Hammarsten","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Sahlgrenska Academy at the University of Gothenburg  Gothenburg Sweden"},{"name":"Laboratory of Clinical Chemistry Sahlgrenska University Hospital  Gothenburg 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