{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T12:49:41Z","timestamp":1779281381413,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Myelodysplastic syndromes (MDS) can present a challenge for clinicians. Multicolor flow cytometry (MFC) can aid in establishing a diagnosis. The aim of this study was to determine the optimal MFC approach for MDS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 102 MDS (39 low\u2010grade MDS), 83 cytopenic patients without myeloid neoplastic disorders (control group), and 35 healthy donors. Bone marrow was analyzed using a six\u2010color MFC. Analysis was conducted according to the \u201cOgata score,\u201d \u201cWells score,\u201d and the integrated flow cytometry (iFC) score.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The respective sensitivity and specificity values were 77.5% and 90.4% for the Ogata score, 79.4% and 81.9% for the Wells score, and 87.3% and 87.6% for the iFC score. Specificity was not 100% due to deviations of MFC parameters in the control group. Patients with paroxysmal nocturnal hemoglobinuria (PNH) had higher levels of CD34<jats:sup>+<\/jats:sup>CD7<jats:sup>+<\/jats:sup> myeloid cells than donors. Aplastic anemia and PNH were characterized by a high proportion of CD56<jats:sup>+<\/jats:sup> cells among CD34<jats:sup>+<\/jats:sup> precursors and neutrophils. The proportion of MDS\u2010related features increased with the progression of MDS. The highest number of CD34<jats:sup>+<\/jats:sup> blasts was found in MDS with excess blasts. MDS with isolated del(5q) was characterized by a high proportion of CD34<jats:sup>+<\/jats:sup>CD7<jats:sup>+<\/jats:sup> cells and low granularity of neutrophils. In 39 low\u2010grade MDS, the sensitivities were 53.8%, 61.5%, and 71.8% for Ogata score, Wells score, and iFC, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results support iFC as a useful diagnostic tool in MDS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21965","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T13:26:09Z","timestamp":1602681969000},"page":"312-321","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Diagnostic significance of flow cytometry scales in diagnostics of myelodysplastic syndromes"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5932-0285","authenticated-orcid":false,"given":"Yulia O.","family":"Davydova","sequence":"first","affiliation":[{"name":"Laboratory of Immunophenotyping National Research Center for Hematology  Moscow 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