{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:48:27Z","timestamp":1782470907217,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>B\u2010lymphoblastic leukemia (B\u2010ALL) is the most common childhood malignancy, and its diagnosis requires immunophenotypically demonstrating blast B cell lineage differentiation. Expression of myeloperoxidase (MPO) in B\u2010ALL is well\u2010described and it has been recognized that a diagnosis of mixed phenotype acute leukemia should be made cautiously if MPO expression is the sole myeloid feature in these cases. We sought to determine whether MPO expression in pediatric B\u2010ALL was associated with differences in laboratory, immunophenotypic, or clinical features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We reviewed clinical, diagnostic bone marrow flow cytometry, and laboratory data for all new B\u2010ALL diagnoses at our pediatric institution in 5\u2009years. Cases were categorized as MPO positive (MPO+) or negative (MPO\u2212) using a threshold of \u226520% blasts expressing MPO at intensity greater than the upper limit of normal lymphocytes on diagnostic bone marrow flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 148 cases were reviewed, 32 of which (22%) were MPO+. MPO+ B\u2010ALL was more frequently hyperdiploid and less frequently harbored <jats:italic>ETV6\u2010RUNX1<\/jats:italic>; no MPO+ cases had <jats:italic>KMT2A<\/jats:italic> rearrangements or <jats:italic>BCR\u2010ABL1<\/jats:italic>. Although not significantly so, MPO+ B\u2010ALL was less likely than MPO\u2212 B\u2010ALL to have positive end\u2010of\u2010induction minimal residual disease studies (9.4 and 24%, respectively), but relapse rates and stem cell transplantation rates were similar between groups. Aberrant expression of other more typically myeloid markers was similar between these groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In our study cohort, MPO+ B\u2010ALL showed minimal residual disease persistence less often after induction chemotherapy but otherwise had similar clinical outcomes to MPO\u2212 B\u2010ALL, with similar rates of additional myeloid antigen aberrancy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21966","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T19:15:43Z","timestamp":1602616543000},"page":"446-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Clinical and laboratory features associated with myeloperoxidase expression in pediatric B\u2010lymphoblastic leukemia"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-5471","authenticated-orcid":false,"given":"Eric","family":"McGinnis","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Au","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"},{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Morrison","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"},{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate M.","family":"Chipperfield","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"},{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audi F.","family":"Setiadi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"},{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorraine","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Tsang","sequence":"additional","affiliation":[{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Vercauteren","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver Canada"},{"name":"Division of Hematopathology British Columbia Children's Hospital  Vancouver Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428199809058390"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1309\/HDUE-DN2E-B72E-JK99"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428199409049717"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/110.5.575"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21518"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12277"},{"key":"e_1_2_7_9_1","first-page":"111","article-title":"Comparison of myeloperoxidase detection by flow cytometry using two different clones of monoclonal antibodies","volume":"26","author":"Leong C. F.","year":"2004","journal-title":"The Malaysian Journal of Pathology"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx021"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-09-807602"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPZ7E9IMXVSNPT"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21613"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401305"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.1.18"},{"key":"e_1_2_7_16_1","first-page":"657","article-title":"Flow cytometric analysis of human bone marrow. III. Neutrophil Maturation","volume":"4","author":"Terstappen L.","year":"1990","journal-title":"Leukemia"},{"key":"e_1_2_7_17_1","first-page":"1180","article-title":"Expression of myeloperoxidase mRNA by leukemic cells from childhood acute lymphoblastic leukemia","volume":"7","author":"Zhou M.","year":"1993","journal-title":"Leukemia"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21966","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21966","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21966","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T07:06:56Z","timestamp":1693292816000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,13]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1002\/cyto.b.21966"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21966","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,13]]},"assertion":[{"value":"2020-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}