{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:28:26Z","timestamp":1790044106222,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T00:00:00Z","timestamp":1607040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H04286"],"award-info":[{"award-number":["17H04286"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The frequency of tumor cell dissemination in the peritoneal cavity is critically related to the progression of peritoneal metastases (PM). Recently, flow cytometry (FCM) has been successfully used to detect tumor cells in malignant effusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 143 single cell suspensions derived from ascites or peritoneal lavages from patients with advanced gastric cancer (GC) were stained with monoclonal antibodies to CD45 and to CD326 as well as 4,6\u2010diamidino\u20102\u2010phenylindole (DAPI) and FVS780. Using FCM, tumor\u2010leukocyte ratio (TLR) were calculated from CD45(\u2212)CD326(+) tumor cell counts\/ CD45(+)CD326(+) leukocyte counts in DAPI (+) FVS780(\u2212) gated area. In 54 patients, the ratios of CD11b(+), CD4(+) and CD8(+) cells in CD45(+) leukocytes were evaluated in parallel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TLR of 69 patients with PM were significantly higher than those of 74 without PM (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and log(TLR) showed strong correlation with peritoneal cancer index scores in 51\u2009PM (+) patients (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.439). TLR in PM (+) patients also correlated with the ratio of CD11b (+) myeloid cells (<jats:italic>r<\/jats:italic>\u00a0=\u20090.547), and correlated inversely with those of CD4(+) (<jats:italic>r<\/jats:italic>\u00a0=\u2009\u22120.490) and CD8(+) T cells (<jats:italic>r<\/jats:italic>\u00a0=\u2009\u22120.648). In PM (\u2212) patients who underwent gastrectomy, TLR never exceeded 0.1% in patients with primary GC without serosal involvement (&lt;T4). However, TLR in patients with T4 GC were significantly higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05) and peritoneal recurrence occurred in 6\/8 patients whose TLR exceeded 0.1%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TLR in peritoneal fluid reflects tumor burden and the immune environment in peritoneal cavity. Multicolor FCM may provide additional information which can be used for the treatment of the patients with PM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21978","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T07:43:45Z","timestamp":1607154225000},"page":"666-675","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Flow cytometry\u2010based analysis of tumor\u2010leukocyte ratios in peritoneal fluid from patients with advanced gastric cancer"],"prefix":"10.1002","volume":"100","author":[{"given":"Kazuya","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Kurashina","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rihito","family":"Kanamaru","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Ohzawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironori","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyo","family":"Miyato","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Hosoya","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan Kawarai","family":"Lefor","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Sata","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5708-7130","authenticated-orcid":false,"given":"Joji","family":"Kitayama","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21258"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603494"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(99)00162-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.10.3406"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800830526"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-008-0483-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20707"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2015.06.003"},{"key":"e_1_2_7_11_1","first-page":"2875","article-title":"Ovarian cancer\u2010associated ascites demonstrates altered immune environment: Implications for antitumor immunity","volume":"29","author":"Giuntoli R. 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