{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T15:10:48Z","timestamp":1771600248572,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T00:00:00Z","timestamp":1608768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Flow cytometry immunophenotyping (FCIP) can improve diagnosis of myelodysplastic syndromes (MDS) and myelodysplastic\/myeloproliferative neoplasms (MDS\/MPN), although its application is challenging due to difficulties in standardization, complexity of antibody panels and subjective interpretation of data. Since blasts are invariably affected in these disorders, we developed a FCIP approach for detailed and objective analysis of the blast population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>FCIP using a one\u2010tube 10\u2010color (13\u2010marker) antibody panel was performed on bone marrow samples from 23 MDS and 8 MDS\/MPN patients, 21 cytopenic patients non\u2010diagnostic for MDS (Non\u2010MDS), and 16 Control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MDS and MDS\/MPN cases demonstrated one to several immunophenotypic abnormalities including: increased myeloblasts, decreased stage\u20101 hematogones, aberrant stem cells, abnormal myeloblast heterogeneity\/divergence from normal, increased or decreased CD45 intensity, increased CD117 or CD123 intensity, decreased CD38 intensity, and aberrant expression of lineage markers (CD5, CD19, CD56). A Blast score was developed that showed sensitivity of 80.6% and specificity of 90.5% for immunophenotypic diagnosis of MDS and MDS\/MPN. Expression levels of CD45RA and CD371 were used to evaluate abnormal myeloblast heterogeneity and stem cell aberrancy. Both these features were, for the first time, incorporated into a scoring system and resulted in 19% increase in the sensitivity of the assay for lower\u2010risk MDS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Deep immunophenotypic analysis of the blast population is valuable for diagnosis of MDS and MDS\/MPN and can potentially provide sensitivity and specificity figures comparable to those previously described using more comprehensive panels that assess maturing myelomonocytic and erythroid elements in addition to progenitor cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21983","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T09:33:29Z","timestamp":1608888809000},"page":"574-589","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Exploring blast composition in myelodysplastic syndromes and myelodysplastic\/myeloproliferative neoplasms: <scp>CD45RA<\/scp> and <scp>CD371<\/scp> improve diagnostic value of flow cytometry through assessment of myeloblast heterogeneity and stem cell aberrancy"],"prefix":"10.1002","volume":"100","author":[{"given":"Afshin","family":"Shameli","sequence":"first","affiliation":[{"name":"Division of Hematology, Alberta Precision Laboratories, South Zone, Department of Pathology &amp; Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poonam","family":"Dharmani\u2010Khan","sequence":"additional","affiliation":[{"name":"Division of Hematology, Alberta Precision Laboratories, South Zone, Department of Pathology &amp; Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Luider","sequence":"additional","affiliation":[{"name":"Division of Hematology, Alberta Precision Laboratories, South Zone, Department of Pathology &amp; Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iwona","family":"Auer","sequence":"additional","affiliation":[{"name":"Division of Hematology, Alberta Precision Laboratories, South Zone, Department of Pathology &amp; Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meer\u2010Taher","family":"Shabani\u2010Rad","sequence":"additional","affiliation":[{"name":"Division of Hematology, Alberta Precision Laboratories, South Zone, Department of Pathology &amp; 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