{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:03:46Z","timestamp":1769601826105,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,7]],"date-time":"2021-03-07T00:00:00Z","timestamp":1615075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Multi\u2010parametric flow cytometry (MFC) is a helpful tool for detecting neoplastic cells in malignant lymphoma; however, lymphoma cells can be difficult to detect when characteristic immunophenotypic abnormalities are not evident. We evaluated the stainability of VS38, which is used for multiple myeloma, in normal and abnormal B cells using MFC to develop a new strategy for detecting lymphoma cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared the median fluorescence intensity of VS38 staining in lymphocytes from patients without hematopoietic neoplasms and in B cells from 26 patients with B cell lymphoma (BCL). To evaluate the performance of VS38 gating, we compared VS38\u2010positive B cells with the percentages of BCL cells, and with the mutation ratios of <jats:italic>MYD88<\/jats:italic> L265P measured by droplet digital PCR in patients with lymphoplasmacytic lymphoma (LPL)\/Waldenstr\u00f6m macroglobulinemia (WM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD27\u2010positive memory B cells were stained with VS38, whereas normal lymphocytes were faintly stained. Lymphoma cells were stained with VS38 in 11 of 12 patients with LPL\/WM, 3 of 3 with chronic lymphocytic leukemia, 3 of 5 with mantle cell lymphoma, 2 of 4 with follicular lymphoma, and 1 of 1 with splenic marginal zone lymphoma. The percentages of VS38\u2010positive B cells in VS38\u2010positive BCL were equivalent to those of lymphoma cells and the mutation ratios of <jats:italic>MYD88<\/jats:italic> L265P in LPL\/WM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>VS38 identified neoplastic cells in plasma cell disorders and BCL. This might improve the accuracy of BCL diagnosis, especially in patients with LPL\/WM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22000","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T06:55:54Z","timestamp":1615186554000},"page":"50-61","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>VS38<\/scp> staining contributes to a novel gating strategy in flow cytometry for small B cell lymphoma, especially in lymphoplasmacytic lymphoma\/Waldenstr\u00f6m macroglobulinemia"],"prefix":"10.1002","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2688-8522","authenticated-orcid":false,"given":"Shumpei","family":"Mizuta","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"},{"name":"Laboratory of Hematology, Division of Medical Biophysics Kobe University Graduate School of Health Sciences  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Yamane","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saya","family":"Mononobe","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asami","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichiro","family":"Matsuki","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Komai","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Koba","sequence":"additional","affiliation":[{"name":"Department of Hematology Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Mitani","sequence":"additional","affiliation":[{"name":"Department of Hematology Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahito","family":"Kawata","sequence":"additional","affiliation":[{"name":"Department of Hematology Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"},{"name":"Department of Hematology and Oncology Graduate School of Medicine, Kyoto University  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Tamekane","sequence":"additional","affiliation":[{"name":"Department of Hematology Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsumasa","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Hematology Hyogo Prefectural Amagasaki General Medical Center  Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.1997.d01-599.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1309\/PMQXVY619Q8Y43AR"},{"key":"e_1_2_7_4_1","first-page":"221","article-title":"VS38 identifies myeloma cells with dim CD38 expression and plasma cells dollowing daratumumab therapy, which interferes with CD38 detection for 4 to 6 months","volume":"153","author":"Courville E. L.","year":"2020","journal-title":"American Journal of Clinical Pathology"},{"key":"e_1_2_7_5_1","first-page":"326","article-title":"Distinction between small lymphocytic and mantle cell lymphoma by immunoreactivity for CD23","volume":"7","author":"Dorfman D. M.","year":"1994","journal-title":"Modern Pathology"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-09-525808"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.62"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA117.000539"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-019-02730-x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12909"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-015-8688-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-019-02685-z"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-08-174961"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12979-019-0159-6"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.124"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2009.01.017"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPEBK31VQQNDDR"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.15584"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21756"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(97)80038-6"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-01-550905"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200710"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.5.418"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-09-457101"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21844"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-09-454355"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1309\/T88X-71U4-WC0R-2531"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.22000","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:55:01Z","timestamp":1693220101000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,7]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/cyto.b.22000"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22000","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,7]]},"assertion":[{"value":"2020-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}