{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:45:39Z","timestamp":1790829939502,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T00:00:00Z","timestamp":1623456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006687","name":"Universit\u00e9 Claude Bernard Lyon 1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006687","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006451","name":"Hospices Civils de Lyon","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006451","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA\u2010DR), measured by standardized flow cytometry procedure, is a reliable indicator of immunosuppression in severely injured intensive care unit patients. As such, it is used as stratification criteria in clinical trials evaluating novel immunostimulating therapies. Preanalytical constraints relative to the short delay between blood sampling and flow cytometry staining have nevertheless limited its use in multicentric studies. The objective of the present work was to compare mHLA\u2010DR expression between whole blood samples simultaneously drawn in EDTA or Cyto\u2010Chex BCT tubes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In two university hospitals, mHLA\u2010DR was assessed in fresh whole blood from septic patients (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u200912) and healthy donors (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u20096) simultaneously sampled on EDTA and Cyto\u2010Chex BCT tubes. Staining was performed immediately after sampling and after blood storage at room temperature.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We confirmed that samples collected in Cyto\u2010Chex tube had substantially enhanced stability for mHLA\u2010DR results (48\u201372\u2009h) over those collected in EDTA. On baseline values, despite good correlation between tubes (\n                      <jats:italic>r<\/jats:italic>\n                      \u00a0=\u20090.98,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u20090.001), mHLA\u2010DR expression was systematically lower with Cyto\u2010Chex BCT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present reports confirms the potential of Cyto\u2010Chex BCT tubes to stabilize mHLA\u2010DR expression before staining and extends the work of Quadrini et al. [Cytometry B 2021;100:103\u2010114]. In centers without rapid access to flow cytometry facilities, it enables to tolerate delays in mHLA\u2010DR staining. However, a 30% gap exists between results obtained with EDTA and Cyto\u2010Chex BCT tubes. As current thresholds for clinical decisions were obtained with EDTA samples, further studies are needed to confirm clinical thresholds with Cyto\u2010Chex BCT tubes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cyto.b.22025","type":"journal-article","created":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T07:10:43Z","timestamp":1623481843000},"page":"384-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Bicentric evaluation of stabilizing sampling tubes for assessment of monocyte\n                    <scp>HLA\u2010DR<\/scp>\n                    expression in clinical samples"],"prefix":"10.1002","volume":"102","author":[{"given":"Sarah","family":"Hamada","sequence":"first","affiliation":[{"name":"Hospices Civils de Lyon, Edouard Herriot Hospital, Immunology Laboratory  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Jeannet","sequence":"additional","affiliation":[{"name":"Inserm CIC 1435 Dupuytren Teaching Hospital  Limoges France"},{"name":"UMR CNRS 7276, INSERM 1262, Faculty of Medicine University of Limoges  Limoges France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgane","family":"Gossez","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Edouard Herriot Hospital, Immunology Laboratory  Lyon France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon Universit\u00e9 Claude Bernard\u2010Lyon 1 Team \u201cNLRP3 inflammation and immune response to sepsis\u201d  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Cour","sequence":"additional","affiliation":[{"name":"Medical intensive Care Department Hospices Civils de Lyon, Edouard Herriot Hospital  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Argaud","sequence":"additional","affiliation":[{"name":"Medical intensive Care Department Hospices Civils de Lyon, Edouard Herriot Hospital  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2531-1652","authenticated-orcid":false,"given":"Bruno","family":"Francois","sequence":"additional","affiliation":[{"name":"Inserm CIC 1435 Dupuytren Teaching Hospital  Limoges France"},{"name":"Dupuytren Teaching Hospital Medical\u2010Surgical Intensive Care Unit  Limoges France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Daix","sequence":"additional","affiliation":[{"name":"Inserm CIC 1435 Dupuytren Teaching Hospital  Limoges France"},{"name":"Dupuytren Teaching Hospital Medical\u2010Surgical Intensive Care Unit  Limoges France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0462-4235","authenticated-orcid":false,"given":"Fabienne","family":"Venet","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Edouard Herriot Hospital, Immunology Laboratory  Lyon France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon Universit\u00e9 Claude Bernard\u2010Lyon 1 Team \u201cNLRP3 inflammation and immune response to sepsis\u201d  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9961-5739","authenticated-orcid":false,"given":"Guillaume","family":"Monneret","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Edouard Herriot Hospital, Immunology Laboratory  Lyon France"},{"name":"EA 7426 \u201cPathophysiology of Injury\u2010Induced Immunosuppression\u201d (Universit\u00e9 Claude Bernard Lyon 1 \u2010 Hospices Civils de Lyon \u2010 bioM\u00e9rieux), Joint Research Unit HCL\u2010bioM\u00e9rieux  Lyon 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