{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T07:30:31Z","timestamp":1776929431782,"version":"3.51.2"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T00:00:00Z","timestamp":1631750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hereditary spherocytosis (HS) is the most common inherited hemolytic anemia. The flow cytometric test using eosin\u20105\u2032maleimide (EMA) is a well\u2010established diagnostic method. However, in order to improve HS detection, it is recommended that EMA and an osmotic fragility test (OFT) both be performed. OFT is time consuming and labor intensive. We used a flow cytometric (FOFT) adaptation of the classical OFT reported by Yamamoto. We compare the FOFT to the classical OFT including practical data and propose options for simplifying this method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Suspected and known HS patients and controls were tested by the following methods: EMA, OFT, and FOFT including some modifications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The FOFT method is robust and correlates to loss of red blood cells. OFT and FOFT gave similar results in healthy controls and four HS patients. Normal range for FOFT in 70 adults is shown and can be used as a reference value. Neonates should have their own normal range defined. Overnight sample incubation at 37\u00b0C did not add information to the FOFT results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our modified Yamomoto FOFT can replace the classic OFT as the addition to EMA for the diagnosis of HS. The use of flow cytometry in both these methods requires small sample volume, is reproducible, simpler, and produces results more rapidly.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22032","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T07:39:32Z","timestamp":1631777972000},"page":"377-383","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of a modified flow cytometry osmotic fragility test with the classical method for the diagnosis of hereditary spherocytosis"],"prefix":"10.1002","volume":"102","author":[{"given":"Yael","family":"Shahal\u2010Zimra","sequence":"first","affiliation":[{"name":"Hematology Laboratory, flow cytometry unit Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuval","family":"Nosgorodcky","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, flow cytometry unit Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Eshel","sequence":"additional","affiliation":[{"name":"Hematology and Blood Bank Laboratories Ziv Medical Center associated with the Bar Ilan University Faculty of Medicine  Safed Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohar","family":"Rotem","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, flow cytometry unit Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liron","family":"Ross","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, flow cytometry unit Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itay","family":"Pickholtz","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, flow cytometry unit Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Rabizadeh","sequence":"additional","affiliation":[{"name":"Felsenstein Medical Research Center, Sackler School of Medicine Tel Aviv University Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Chezar","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, flow cytometry unit Rabin Medical Center Beilinson Hospital, Rabin Medical Center  Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12794"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.052845"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08921.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21511"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-010-1112-0"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24157"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700460214"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01715351"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/1110-1067.148229"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06944.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21187"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12533"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0141-9854.2003.00557.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02416.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12070"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPO7V4OGXLIIPP"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61588-3"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.d01-2074.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16750"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20583"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20448"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/2211068214532254"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22032","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.22032","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:16:07Z","timestamp":1692778567000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,16]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1002\/cyto.b.22032"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22032","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,16]]},"assertion":[{"value":"2021-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}