{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T19:12:33Z","timestamp":1775675553472,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 CA008748"],"award-info":[{"award-number":["P30 CA008748"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lineage infidelity is characteristic of mixed phenotype acute leukemia and is also seen in blast phase of chronic myeloid leukemia (CML), myeloid\/lymphoid neoplasia with eosinophilia and gene rearrangements, and subtypes of acute myeloid leukemia. Driver genetic events often occur in multipotent progenitor cells in myeloid neoplasms, suggesting that multilineage output may be more common than appreciated. This phenomenon is not well studied in myelodysplastic syndrome (MDS) and non\u2010CML myeloproliferative neoplasms (MPN).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We systematically evaluated phenotypic lineage infidelity by reviewing bone marrow pathology and flow cytometry (FC) studies of 1262 consecutive patients with a diagnosis of MDS and\/or non\u2010CML MPN. We assessed B\u2010 and T\u2010cells in these patients by FC. When abnormal B\u2010lymphoblast (ABLB) populations were detected, we additionally evaluated immature B\u2010cells using a high sensitivity FC assay for B\u2010lymphoblastic leukemia\/lymphoma (B\u2010ALL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 9 patients (7 MDS, 7\/713, 1%; 2 non\u2010CML MPN, 2\/312, 0.6%; 0 in MDS\/MPN) with low\u2010level ABLB populations (0.012%\u20133.6% of WBCs in marrow) with abnormal immunophenotypes. Genetic studies on flow sorted cell populations confirmed that some ABLB populations were clonally related to myeloid blasts (4\/6, 67%). On follow\u2010up, ABLB populations in 8\/9 patients remained stable or disappeared. Only 1 case progressed to B\u2010ALL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings demonstrate that phenotypically detectable abnormal immature B lineage output occurs in MDS and non\u2010CML MPN, albeit rarely. While presence of ABLB does not necessarily reflect blast crisis, the underlying disease biology of our findings may ultimately be relevant to patient management and warrants further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22047","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T09:30:17Z","timestamp":1639387817000},"page":"243-252","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Abnormal B\u2010lymphoblasts in myelodysplastic syndromes and myeloproliferative neoplasms other than chronic myeloid leukemia"],"prefix":"10.1002","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5884-8424","authenticated-orcid":false,"given":"Alexander","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyadarshini","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeeyeon","family":"Baik","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison","family":"Sigler","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dory","family":"Londono","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cytogenetics Laboratory Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"},{"name":"Department of Pathology Molecular Diagnostic Laboratory, Memorial Sloan Kettering Cancer Center  New York City NewYork USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria E.","family":"Arcila","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"},{"name":"Department of Pathology Molecular Diagnostic Laboratory, Memorial Sloan Kettering Cancer Center  New York City NewYork USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Dogan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cytogenetics Laboratory Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikhail","family":"Roshal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Service Memorial Sloan Kettering Cancer Center  New York City New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"issue":"3","key":"e_1_2_8_2_1","first-page":"322","article-title":"Transformation of the 5q\u2010 syndrome to acute lymphoblastic leukemia: A report of two cases and review of the literature","volume":"4","author":"Agostino N. 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