{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T06:10:42Z","timestamp":1769926242916,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T00:00:00Z","timestamp":1654300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008459","name":"University of Calgary","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008459","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>CD200 (OX\u20102) is a valuable marker in the diagnosis of B\u2010cell neoplasms and is commonly used in the screening panels for assessment of peripheral blood B\u2010cell lymphoproliferative disorders. However, there is limited understanding about CD200 expression in T\u2010cell neoplasms. A previous study has shown that CD200 is expressed on the neoplastic cells of angioimmunoblastic T\u2010cell lymphoma (AITL) by immunohistochemistry, but no study has evaluated CD200 expression in T\u2010cell neoplasms by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We assessed CD200 expression in peripheral blood T\u2010cell lymphoproliferative disorders by retrospective analysis of our institutional flow cytometry screening database over a 6\u2010year period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In addition to AITL, we identified CD200 expression in a significant number of mycosis fungoides\/Sezary syndrome cases (58%, 19 of 33 samples), while most other T\u2010cell neoplasms were negative for CD200. These findings were confirmed by CD200 immunohistochemical staining of tissue specimens from our patient cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CD200 is commonly expressed on circulating Sezary cells, a feature that can potentially improve the diagnostic value of flow cytometry for assessment of T\u2010cell neoplasms.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22080","type":"journal-article","created":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T11:04:57Z","timestamp":1654340697000},"page":"303-311","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>CD200<\/scp> expression on Sezary cells: A valuable tool for flow cytometric assessment of peripheral blood T\u2010cell neoplasms"],"prefix":"10.1002","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4614-4291","authenticated-orcid":false,"given":"Afshin","family":"Shameli","sequence":"first","affiliation":[{"name":"Division of Hematopathology, Alberta Precision Laboratories, South Zone and Department of Pathology and Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tariq M.","family":"Roshan","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Alberta Precision Laboratories, South Zone and Department of Pathology and Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3747\/co.26.4593"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000258"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018022608"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2012.2089"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPBV9ELXC0ECVL"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.68"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP38XRRUGSQOVC"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31820065c9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22041"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21936"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21878"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21963"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21933"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx041"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00404426"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.124"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.356"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-029355"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0602"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPEBK31VQQNDDR"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21888"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21785"},{"key":"e_1_2_7_24_1","volume-title":"WHO classification of Tumours of Haematopoietic and lymphoid tissues","author":"Swerdlow S. 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