{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T17:18:59Z","timestamp":1786555139444,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>ZNF384<\/jats:italic> rearrangement has been recently identified as a new subtype of B\u2010cell precursor acute lymphoblastic leukemia (BCP\u2010ALL). However, comprehensive studies clarifying immunophenotypic features and discriminating them from non\u2010<jats:italic>ZNF384<\/jats:italic> in adult BCP\u2010ALL remain scarce to date.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Flow cytometric assessments were retrospectively performed in 43 patients with <jats:italic>ZNF384<\/jats:italic> rearrangement, 45 with <jats:italic>BCR\u2010ABL1<\/jats:italic>, 29 with <jats:italic>KMT2A<\/jats:italic> rearrangement and 44 with other BCP\u2010ALL in the analysis cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD33\u2010 and CD13\u2010positive frequencies were significantly higher in patients with <jats:italic>ZNF384<\/jats:italic> rearrangement than in those with non\u2010<jats:italic>ZNF384<\/jats:italic>; however, no significant difference was observed in CD10\u2010 and CD123\u2010positive frequencies. Analysis of antigen\u2010positive cell proportion and median fluorescence intensity (MFI) further indicated that patients with <jats:italic>ZNF384<\/jats:italic> rearrangement had significantly lower CD10 and higher CD33, CD13, and CD123 proportion and MFI. However, compared with <jats:italic>KMT2A<\/jats:italic> rearrangement, the CD10 expression in patients with <jats:italic>ZNF384<\/jats:italic> rearrangement was higher, with the median percentage and MFI of 36.16 (3.63\u201394.79)% versus 4.53 (0.03\u201321.00)%, and 4.50 (0.86\u201332.26) versus 2.06 (0.87\u20134.04), respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Furthermore, compared with <jats:italic>BCR\u2010ABL1<\/jats:italic> and other BCP\u2010ALL, <jats:italic>ZNF384<\/jats:italic> rearrangement had significantly higher CD33 and CD13 proportion and MFI (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, respectively). In addition, higher CD123 proportion and MFI in <jats:italic>ZNF384<\/jats:italic> rearrangement than those in the other three groups were reported for the first time (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). A flow cytometry scoring system, including CD10%, CD33MFI, CD13%, and CD123MFI, was proposed and verified to predict <jats:italic>ZNF384<\/jats:italic> rearrangement with high sensitivity and specificity, that is, 76.74% and 91.53% in the analysis and 87.50% and 91.30% in the validation cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The multiparameter immunophenotypic scoring system could suggest <jats:italic>ZNF384<\/jats:italic> rearrangement.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22086","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T10:37:29Z","timestamp":1655980649000},"page":"360-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Immunophenotypic characteristics of <scp><i>ZNF384<\/i><\/scp> rearrangement compared with <scp><i>BCR\u2010ABL1<\/i><\/scp>, <scp><i>KMT2A<\/i><\/scp> rearrangement, and other adult <scp>B\u2010cell<\/scp> precursor acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"102","author":[{"given":"Ya\u2010Zhe","family":"Wang","sequence":"first","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation National Clinical Research Center for Hematologic Disease  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Zhen","family":"Qin","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation National Clinical Research Center for Hematologic Disease  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chang","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation National Clinical Research Center for Hematologic Disease  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Ying","family":"Yuan","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation National Clinical Research Center for Hematologic Disease  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital, Peking University Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation National Clinical Research Center for Hematologic Disease  Beijing 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