{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:00Z","timestamp":1786977480996,"version":"build-2736575974"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bronchoalveolar (BAL) cellular analysis can be supportive in the diagnosis of interstitial lung disease. The flow cytometric analysis of BAL fluid cells is complicated by cell fragility and adherence and autofluorescence of macrophages, making conventional analysis of BAL fluid cells as done in external quality schemes (EQA) for blood lymphocyte subsets, not representative. Following a procedure for stabilized BAL cells, a separate EQA was set up. The results of 20\u2009years' experience are presented.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From each round between 2000 and 2020 the following flow cytometric parameters were recorded from each participant: total lymphocyte population (TLY), CD3+ lymphocytes, CD3+\u2009CD4+ lymphocytes, CD3+\u2009CD8+ lymphocytes, CD3\u2212 CD16+\/56+ lymphocytes, CD19+ lymphocytes and CD103\u2009+\u2009CD3+ lymphocytes. In addition, the eosinophils and neutrophils were recorded. The mean and standard deviation of each parameter per round were calculated. The 40 rounds were divided in four respective groups of 10 in order to compare the results as function of time. In addition the interpretation of the results of participants was scored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The median SD in the four groups was below 10% for all parameters except for TLY and the CD103+\u2009CD3+\u2009lymphocytes. No improvement in time was observed for any (sub)population except for the CD3+\u2009CD4+ subset. Interpretation of the results varied based on disease, with greatest consensus for sarcoidosis cases and lowest for nonspecific interstitial lung disease cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A dedicated EQA for BAL fluid cellular analysis appears to be justified as the test material is substantially different from that of peripheral blood. We show that adequate analytical and post\u2010analytical quality control can be achieved.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cyto.b.22090","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T10:03:44Z","timestamp":1662545024000},"page":"451-457","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["External quality assessment of flow cytometric bronchoalveolar lavage cellular analysis: 20\u2009years' experience in\n                    <scp>The Netherlands<\/scp>"],"prefix":"10.1002","volume":"102","author":[{"given":"A. H. Leontine","family":"Mulder","sequence":"first","affiliation":[{"name":"Medlon B.V., Department of Clinical Chemistry and Laboratory Medicine, Enschede and Clinical Chemistry Ziekenhuis Groep Twente  Almelo The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7880-2615","authenticated-orcid":false,"given":"Harrie H. M.","family":"Eidhof","sequence":"additional","affiliation":[{"name":"Medlon B.V., Department of Clinical Chemistry and Laboratory Medicine Ziekenhuis Groep Twente  Enschede The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan W.","family":"Gratama","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Erasmus MC  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21531"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.2001.00356.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10151"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.310.6973.170"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20154"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21883"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2018.08.003"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.3.271-277.1998"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20351"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20370"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201202-0320ST"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21266"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2016-0970"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2019-0191ST"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2011.011128"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.22090","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T22:48:02Z","timestamp":1692658082000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,7]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1002\/cyto.b.22090"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22090","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,7]]},"assertion":[{"value":"2022-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}