{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:15:28Z","timestamp":1789593328123,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T00:00:00Z","timestamp":1669420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Multiparametric flow cytometry (MFC) has become a powerful tool in minimal residual disease (MRD) detection in B\u2010lymphoblastic leukemia\/lymphoma (B\u2010ALL). In the setting of targeted immunotherapy, B\u2010ALL MRD detection often relies on alterative gating strategies, such as the utilization of CD22 and CD24. It is important to depict the full diversity of normal cell populations included in the alternative B\u2010cell gating methods to avoid false\u2010positive results. We describe two CD22\u2010positive non\u2010neoplastic cell populations in the peripheral blood (PB), including one progenitor population of uncertain lineage and one mature B\u2010cell population, which are immunophenotypic mimics of B\u2010ALL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using MFC, we investigated the prevalence and phenotypic profiles of both CD22\u2010positive populations in 278 blood samples from 52 patients with B\u2010ALL; these were obtained pre\u2010 and post\u2010treatment with CD19 and\/or CD22 CAR\u2010T therapies. We further assessed whether these two populations in the blood were exclusively associated with B\u2010ALL or recent anticancer therapies, by performing the same analysis on patients diagnosed with other hematological malignancies but in long\u2010term MRD remission.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The progenitor population and mature B\u2010cell population were detected at low levels in PB of 61.5% and 44.2% of B\u2010ALL patients, respectively. Both cell types showed distinctive and highly consistent antigen expression patterns that are reliably distinguishable from B\u2010ALL. Furthermore, their presence is not restricted solely to B\u2010ALL or recent therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings aid in building a complete immunophenotypic profile of normal cell populations in PB, thereby preventing misdiagnosis of B\u2010ALL MRD and inappropriate management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22104","type":"journal-article","created":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T14:39:31Z","timestamp":1669473571000},"page":"294-303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Circulating <scp>CD22<\/scp>+\/<scp>CD19<\/scp>\u2212\/<scp>CD24<\/scp>\u2212 progenitors and <scp>CD22<\/scp>+\/<scp>CD19<\/scp>+\/<scp>CD24<\/scp>\u2212 mature B cells: Diagnostic pitfalls for minimal residual disease detection in <scp>B\u2010lymphoblastic<\/scp> leukemia"],"prefix":"10.1002","volume":"104","author":[{"given":"Ting","family":"Zhou","sequence":"first","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremiah","family":"Karrs","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Truc","family":"Ho","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa","family":"Doverte","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James N.","family":"Kochenderfer","sequence":"additional","affiliation":[{"name":"Surgery Branch, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirali N.","family":"Shah","sequence":"additional","affiliation":[{"name":"Pediatric Oncology Branch, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2601-3665","authenticated-orcid":false,"given":"Constance M.","family":"Yuan","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5408-9253","authenticated-orcid":false,"given":"Hao\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402552"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404992"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-132837"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-03-633685"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2708"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21482"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqaa242"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24420"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.5323"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)62187-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204828"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2014.04.018"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.22719"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1709866"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1709919"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/2643-3230.BCD-21-0095"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2011.559668"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31179"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02458"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPS1K0OHLJYWWV"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21179"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.042937"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70243-8"},{"key":"e_1_2_7_25_1","first-page":"266","article-title":"Treatment 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