{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:50:30Z","timestamp":1790823030179,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017268","name":"Berlin Institute of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Kidney diseases are a major health concern worldwide. Currently there is a large unmet need for novel biomarkers to non\u2010invasively diagnose and monitor kidney diseases. Urinary cells are promising biomarkers and their analysis by flow cytometry has demonstrated its utility in diverse clinical settings. However, up to date this methodology depends on fresh samples, as cellular event counts and the signal\u2010to\u2010noise\u2010ratio deter over time. Here we developed an easy\u2010to\u2010use two\u2010step preservation method for conservation of urine samples for subsequent flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The protocol utilizes a combination of the formaldehyde releasing agent imidazolidinyl urea (IU) and MOPS buffer, leading to gentle fixation of urinary cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The preservation method increases acceptable storing time of urine samples from several hours to up to 6\u2009days. Cellular event counts and staining properties of cells remain comparable to fresh untreated samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Outlook<\/jats:title><jats:p>The hereby presented preservation method facilitates future investigations on flow cytometry of urinary cells as potential biomarkers and may enable broad implementation in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.22117","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T10:51:18Z","timestamp":1678186278000},"page":"417-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Addition of formaldehyde releaser imidazolidinyl urea and <scp>MOPS<\/scp> buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells: A simple, two step conservation method of urinary cells for flow cytometry"],"prefix":"10.1002","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7164-5760","authenticated-orcid":false,"given":"Paul","family":"Freund","sequence":"first","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Skopnik","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Metzke","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Goerlich","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Klocke","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil","family":"Grothgar","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luka","family":"Prskalo","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falk","family":"Hiepe","sequence":"additional","affiliation":[{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin Germany"},{"name":"Department of Rheumatology and Clinical Immunology Charite \u2013 Universital Hospital Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Enghard","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Medical Intensive Care Charit\u00e9 \u2013 Universital Hospital Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"German Rheumatism Research Center Berlin (DRFZ) An Institute of the Leibniz Foundation  Berlin 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