{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:29:57Z","timestamp":1785529797302,"version":"3.56.0"},"reference-count":9,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T00:00:00Z","timestamp":1684540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC1382"],"award-info":[{"award-number":["CRC1382"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB\/TRR 296"],"award-info":[{"award-number":["SFB\/TRR 296"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SPP2306"],"award-info":[{"award-number":["SPP2306"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immune monitoring of patients on a single\u2010cell level is becoming increasingly important in various diseases. Due to the often very limited availability of human specimens and our increased understanding of the immune systems there is an increasing demand to analyze as many markers as possible simultaneously in one panel. Full spectrum flow cytometry is emerging as a powerful tool for immune monitoring since 5\u2010laser instruments enable characterization of 40 parameters or more in a single sample. Nevertheless, even if only machines with fewer lasers are available, development of novel fluorophore families enables increasing panel sizes. Here, we demonstrate that careful panel design enables the use of 31\u2010color panels on a 3\u2010laser Cytek\u00ae Aurora cytometer for analyzing human peripheral blood leukocytes, without the need for custom configuration and using only commercially available fluorochromes. The panel presented here should serve as an example of a 31\u2010fluorochrome combination that can be resolved on a 3\u2010laser full spectrum cytometer and that can be adapted to comprise other (and possibly more) markers of interest depending on the research focus.<\/jats:p>","DOI":"10.1002\/cyto.b.22126","type":"journal-article","created":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T09:14:02Z","timestamp":1684574042000},"page":"367-373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Resolving 31 colors on a standard 3\u2010laser full spectrum flow cytometer for immune monitoring of human blood samples"],"prefix":"10.1002","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0557-3927","authenticated-orcid":false,"given":"Linda","family":"Hammerich","sequence":"first","affiliation":[{"name":"Department of Hepatology and Gastroenterology Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaroslava","family":"Shevchenko","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Knorr","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebke","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alix","family":"Bruneau","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Tacke","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.612801"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2796"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22113"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24290"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21270"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24213"},{"key":"e_1_2_10_8_1","first-page":"1","article-title":"Development of a 43 color panel for the characterization of conventional and unconventional T\u2010cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry","author":"Sahir F.","year":"2020","journal-title":"Cytometry A"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21984"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.24537"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T04:32:31Z","timestamp":1721968351000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,20]]},"references-count":9,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1002\/cyto.b.22126"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22126","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,20]]},"assertion":[{"value":"2023-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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