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Due to low antibody levels, CVID patients receive intravenous or subcutaneous immunoglobulin replacement therapy as treatment. CVID is associated with the chronic activation of granulocytes, including an increased percentage of low\u2010density neutrophils (LDNs). In this study, we examined changes in the percentage of LDNs and the expression of their surface markers in 25 patients with CVID and 27 healthy donors (HD) after in vitro stimulation of whole blood using IVIg. An oxidative burst assay was used to assess the functionality of LDNs. CVID patients had increased both relative and absolute LDN counts with a higher proportion of mLDNs compared to iLDNs, distinguished based on the expression of CD10 and CD16. Immature LDNs in the CVID and HD groups had significantly reduced oxidative burst capacity compared to mature LDNs. Interestingly we observed reduced oxidative burst capacity, reduced expression of CD10 after stimulation of WB, and higher expression of PD\u2010L1 in mature LDNs in CVID patients compared to HD cells. Our data indicate that that the functional characteristics of LDNs are closely linked to their developmental stage. The observed reduction in oxidative burst capacity in mLDNs in CVID patients could contribute to an increased susceptibility to recurrent bacterial infections among CVID patients.<\/jats:p>","DOI":"10.1002\/cyto.b.22150","type":"journal-article","created":{"date-parts":[[2023,11,25]],"date-time":"2023-11-25T01:23:19Z","timestamp":1700875399000},"page":"99-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Phenotype and oxidative burst of low\u2010density neutrophil subpopulations are altered in <scp>common variable immunodeficiency<\/scp> patients"],"prefix":"10.1002","volume":"106","author":[{"given":"Peter","family":"Slanina","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology and Allergology, Faculty of Medicine Masaryk University  Brno Czech Republic"},{"name":"Institute of Clinical Immunology and Allergology St. Anne's University Hospital  Brno Czech 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