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Here we provide clinical flow cytometry laboratories with technical support adapted for the most commonly used cytometers. Step\u2010by\u2010step explanations of the gating strategy developed on whole peripheral blood are presented while underlining the most common difficulties. In a second part, interpretation recommendations of circulating monocyte partitioning from the dedicated French working group \u201cCytHem\u2010LMMC\u201d are shared as well as the main pitfalls, including false positive and false negative cases. The particular flow\u2010defined inflammatory profile is described and the usefulness of the nonclassical monocyte specific marker, namely slan, highlighted. Examples of reporting to the physician with frequent situations encountered when using the monocyte assay are also presented.<\/jats:p>","DOI":"10.1002\/cyto.b.22176","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T11:44:12Z","timestamp":1713959052000},"page":"203-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Technical, gating and interpretation recommendations for the partitioning of circulating monocyte subsets assessed by flow cytometry"],"prefix":"10.1002","volume":"106","author":[{"given":"Sihem","family":"Tarfi","sequence":"first","affiliation":[{"name":"D\u00e9partement d'H\u00e9matologie et Immunologie Biologiques AP\u2010HP, H\u00f4pital Henri Mondor  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Kern","sequence":"additional","affiliation":[{"name":"MLL Munich Leukemia Laboratory, GmbH  Munich 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