{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:43:57Z","timestamp":1792183437203,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017507","name":"Department of Laboratory Medicine and Pathology, University of Washington","doi-asserted-by":"publisher","award":["2022GJ05"],"award-info":[{"award-number":["2022GJ05"]}],"id":[{"id":"10.13039\/100017507","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The flow cytometry\u2010based evaluation of TRBC1 expression has been demonstrated as a rapid and specific method for detecting T\u2010cell clones in sCD3\u2010positive TCR\u03b1\u03b2+ mature T\u2010cell lymphoma. The aim of the study was to validate the utility of surface (s) TRBC1 and cytoplastic (cy) TRBC1 assessment in detecting clonality of sCD3\u2010negative peripheral T\u2010cell lymphomas (PTCLs), as well as exploring the existence and characteristics of sCD3\u2010negative clonal T\u2010cell populations with uncertain significance (T\u2010CUS). Evaluation of sTRBC1 and cyTRBC1 were assessed on 61 samples from 37 patients with sCD3\u2010negative PTCLs, including 26 angioimmunoblastic T\u2010cell lymphoma (AITL) patients and 11 non\u2010AITL patients. The sCD3\u2010negative T\u2010CUS were screened from 1602 patients without T\u2010cell malignancy and 100 healthy individuals. Additionally, the clonality of cells was further detected through T\u2010cell gene rearrangement analysis. We demonstrated the monotypic expression patterns of cyTRBC1 in all sCD3\u2010negative PTCLs. Utilizing the cyTRBC1 evaluation assay, we identified a novel and rare subtype of sCD3\u2010negative T\u2010CUS for the first time among 13 out of 1602 (0.8%) patients without T\u2010cell malignancy. The clonality of these cells was further confirmed through T\u2010cell gene rearrangement analysis. This subset exhibited characteristics such as sCD3\u2010cyCD3\u2009+\u2009CD4\u2009+\u2009CD45RO+, closely resembling AITL rather than non\u2010AITL. Further analysis revealed that sCD3\u2010negative T\u2010CUS exhibited a smaller clone size in the lymph node and mass specimens compared to AITL patients. However, the clone size of sCD3\u2010negative T\u2010CUS was significantly lower than that of non\u2010AITL patients in both specimen groups. In conclusion, we validated the diagnostic utility of cyTRBC1 in detecting sCD3\u2010negative T\u2010cell clonality, provided a comprehensive analysis of sCD3\u2010negative T\u2010CUS, and established a framework and provided valuable insights for distinguishing sCD3\u2010negative T\u2010CUS from sCD3\u2010negative PTCLs based on their phenotypic properties and clone size.<\/jats:p>","DOI":"10.1002\/cyto.b.22182","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T10:44:28Z","timestamp":1717152268000},"page":"465-475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>cyTRBC1<\/scp> evaluation rapidly identifies <scp>sCD3<\/scp>\u2010negative peripheral T\u2010cell lymphomas and reveals a novel type of <scp>sCD3<\/scp>\u2010negative T\u2010cell clone with uncertain significance"],"prefix":"10.1002","volume":"106","author":[{"given":"Cong","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Cardiology, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhong","sequence":"additional","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study and Department of Laboratory Medicine, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  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