{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T02:35:43Z","timestamp":1783218943750,"version":"3.54.6"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:00:00Z","timestamp":1725840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC1382"],"award-info":[{"award-number":["CRC1382"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Ha 7431\/3\u20101"],"award-info":[{"award-number":["Ha 7431\/3\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Ta434\/8\u20101"],"award-info":[{"award-number":["Ta434\/8\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The gut\u2010liver axis includes the bidirectional communication between the gut and the liver, and thus covers signals from liver\u2010to\u2010gut and from gut\u2010to\u2010liver. Disruptions of the gut\u2010liver axis have been associated with the progression of chronic liver diseases, including alcohol\u2010related and metabolic dysfunction\u2010associated steatotic liver disease and cholangiopathies. Immune cells and their expression of pattern recognition receptors, activation markers or immune checkpoints might play an active role in the communication between gut and liver. Here, we present a 26\u2010color full spectrum flow cytometry panel for human cells to decipher the role of circulating immune cells in gut\u2010liver communication during the progression of chronic liver diseases in a non\u2010invasive manner, which has been optimized to be used on patient\u2010derived whole blood samples, the most abundantly available clinical material. Our panel focuses on changes in pattern recognition receptors, including toll\u2010like receptors (TLRs) or Dectin\u20101, and also includes other immunomodulatory molecules such as bile acid receptors and checkpoint molecules. Moreover, this panel can be utilized to follow the progression of chronic liver diseases and could be used as a tool to evaluate the efficiency of therapeutic targets directed against microbial mediators or modulating immune cell activation.<\/jats:p>","DOI":"10.1002\/cyto.b.22203","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T05:19:17Z","timestamp":1725945557000},"page":"15-22","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A comprehensive 26\u2010color immunophenotyping panel to study the role of the gut\u2010liver axis in chronic liver diseases"],"prefix":"10.1002","volume":"108","author":[{"given":"Alix","family":"Bruneau","sequence":"first","affiliation":[{"name":"Department of Hepatology &amp; Gastroenterology Charit\u00e9 Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaroslava","family":"Shevchenko","sequence":"additional","affiliation":[{"name":"Department of Hepatology &amp; Gastroenterology Charit\u00e9 Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Tacke","sequence":"additional","affiliation":[{"name":"Department of Hepatology &amp; Gastroenterology Charit\u00e9 Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0557-3927","authenticated-orcid":false,"given":"Linda","family":"Hammerich","sequence":"additional","affiliation":[{"name":"Department of Hepatology &amp; Gastroenterology Charit\u00e9 Universit\u00e4tsmedizin Berlin, Campus Virchow\u2010Klinikum (CVK) and Campus Charit\u00e9 Mitte (CCM)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01273-y"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20422-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.612801"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.725390"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5954437"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27385-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.01.011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00551"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/HEP.0000000000000836"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22126"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.7.4679"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.866040"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jceh.2021.11.011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21270"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.136114"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-023-00771-6"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.15586"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.03.029"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/gtc.13106"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-022-00926-2"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbl2812327"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13251"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.111877"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.12.024"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T11:56:30Z","timestamp":1738238190000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,9]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/cyto.b.22203"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22203","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,9]]},"assertion":[{"value":"2024-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}