{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:22:45Z","timestamp":1775672565464,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HLA\u2010B27 is a major histocompatibility complex (MHC) class I antigen which exhibits strong association (90%) with ankylosing spondylitis. HLA\u2010B27 detection in patients by flow cytometry is a widely used clinical test, performed on many different flow cytometer models. We sought to develop and validate a test conversion protocol for the HLA\u2010B27 test performed on the BD FACSCanto to BD's newer FACSLyric flow cytometers. The development and validation experiments were performed using anti\u2010HLA\u2010B27*FITC\/CD3*PE antibody\u2010stained whole blood patient specimens. The anti\u2010HLA\u2010B27*FITC logarithmic median fluorescence (LMF) results on the BD FACSCanto were converted to median fluorescence intensity (MFI) values on the BD FACSLyric. Clustering of the HLA\u2010B27 positive and negative values, using a 3rd order polynomial equation, resulted in a conversion of the BD FACSCanto cutoff values, negative (&lt;150 LMF) and positive (\u2265160 LMF), to negative (&lt;4530 MFI) and positive (\u22656950 MFI) on the BD FACSLyric. Accuracy was assessed by comparing the flow results obtained on the BD FACSCanto and BD FACSLyric to a molecular PCR based assay. Additional validation parameters (compensation verification, intra\u2010 and inter\u2010assay precision, and instrument comparison) were performed per the recommendations outlined in the Clinical and Laboratory Standards Institute (CLSI) H62 guidelines for validation of flow cytometry assays.<\/jats:p>","DOI":"10.1002\/cyto.b.22206","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T19:20:05Z","timestamp":1726600805000},"page":"67-76","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Converting an <scp>HLA<\/scp>\u2010<scp>B27<\/scp> flow assay from the <scp>BD FACSCanto<\/scp> to the <scp>BD FACSLyric<\/scp>"],"prefix":"10.1002","volume":"108","author":[{"given":"Eugene V.","family":"Ravkov","sequence":"first","affiliation":[{"name":"APUP Institute for Clinical and Experimental Pathology  Salt Lake Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel F.","family":"Ventura","sequence":"additional","affiliation":[{"name":"APUP Institute for Clinical and Experimental Pathology  Salt Lake Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swapna","family":"Gudipaty","sequence":"additional","affiliation":[{"name":"APUP Institute for Clinical and Experimental Pathology  Salt Lake Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8604-2073","authenticated-orcid":false,"given":"David","family":"Ng","sequence":"additional","affiliation":[{"name":"APUP Institute for Clinical and Experimental Pathology  Salt Lake Utah USA"},{"name":"Department of Pathology University of Utah School of Medicine  Salt Lake Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio C.","family":"Delgado","sequence":"additional","affiliation":[{"name":"APUP Institute for Clinical and Experimental Pathology  Salt Lake Utah USA"},{"name":"Department of Pathology University of Utah School of Medicine  Salt Lake Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo","family":"Lin","sequence":"additional","affiliation":[{"name":"APUP Institute for Clinical and Experimental Pathology  Salt Lake Utah USA"},{"name":"Department of Pathology University of Utah School of Medicine  Salt Lake Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22125"},{"key":"e_1_2_6_3_1","unstructured":"(2023).BD\u00ae HLA\u2010B27 Kit IVD http:\/\/static.bd.com\/documents\/eifu\/ZMG_23-2563_EN_B_16.pdf"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60635-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(73)91360-3"},{"key":"e_1_2_6_6_1","volume-title":"Validation of Assays Performed by Flow Cytometry","author":"CLSI","year":"2021"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1423.013"},{"key":"e_1_2_6_8_1","unstructured":"FDA. (2024).Medical Devices; Laboratory Developed Tests https:\/\/www.federalregister.gov\/documents\/2024\/05\/06\/2024-08935\/medical-devices-laboratory-developed-tests"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-010-0126-x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.71.3.184"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.10022"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990220211"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2022-0322-CP"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jalm\/jfab046"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e31820f8c99"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-014-2662-7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20039"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-210770"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21630"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T11:56:48Z","timestamp":1738238208000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,17]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/cyto.b.22206"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22206","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,17]]},"assertion":[{"value":"2024-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}