{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T21:17:19Z","timestamp":1785014239773,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T00:00:00Z","timestamp":1727827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucose\u20106\u2010phosphate dehydrogenase (G6PD) deficiency is a common enzymopathy that affects red blood cells (RBCs) and renders them susceptible to oxidative stress. G6PD deficiency can cause hemolytic anemia, especially after exposure to certain drugs or infections. The diagnosis of G6PD deficiency is usually based on spectrophotometric measurement of enzyme activity, but this method has limitations in heterozygous females and in patients with other hematological disorders. In this study, we evaluated the use of flow cytometry as an alternative method for detecting G6PD deficiency in 514 samples (265 females and 249 males) from a clinical laboratory. We compared the results of flow cytometry with those of spectrophotometry and molecular analysis, and assessed the performance of flow cytometry in different subgroups of patients. We found that flow cytometry was able to identify G6PD deficiency in most cases, with high sensitivity and specificity. Flow cytometry also allowed the quantification of the percentage of G6PD\u2010deficient RBCs, which varied among heterozygous females due to X\u2010chromosome inactivation. Moreover, flow cytometry detected several cases of G6PD deficiency that were missed by spectrophotometry, especially in heterozygous females with normal or subnormal enzyme activity. However, flow cytometry also showed some false negative results, mainly in patients with sickle cell disease. Therefore, flow cytometry is a reliable and efficient tool for screening G6PD deficiency, but some precautions should be taken in interpreting the results in patients with other hematological conditions.<\/jats:p>","DOI":"10.1002\/cyto.b.22207","type":"journal-article","created":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T07:45:01Z","timestamp":1727855101000},"page":"161-171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>Glucose\u20106\u2010phosphate dehydrogenase<\/scp> deficiency detection using fluorocytometric assay: Evaluation after 1\u2009year of clinical implementation"],"prefix":"10.1002","volume":"108","author":[{"given":"M.","family":"Souissi","sequence":"first","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Bera","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Boutet","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Chatellier","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Conte","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Brard","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Boquet","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Pissard","sequence":"additional","affiliation":[{"name":"Genetics Department Henri Mondor  Cr\u00e9teil France"},{"name":"Medicine School University Paris Est Creteil (UPEC)  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lahary","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9357-4542","authenticated-orcid":false,"given":"V.","family":"Bob\u00e9e","sequence":"additional","affiliation":[{"name":"Department of Biological Hematology Rouen University Hospital  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/61.10.1086"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630260008993134"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-10045-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1977.tb00589.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.48.1.9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60073-2"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000527806"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci110870"},{"issue":"4","key":"e_1_2_8_10_1","first-page":"359","article-title":"Studies of Glucose\u20106\u2010phosphate dehydrogenase activity of individual erythrocytes: The Methemoglobin\u2010elution test for identification of females heterozygous for G6PD deficiency","volume":"17","author":"Gall J. 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