{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T12:39:08Z","timestamp":1792931948719,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011095","name":"BD Biosciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011095","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rare acute leukemia (AL) components or subtypes such as blastic plasmacytoid dendritic cell neoplasm (BPDCN) or early T\u2010cell precursor acute Lymphoblastic Leukemia (ETP\u2010ALL) can be difficult to detect by routine flow cytometry due to their immunophenotypes overlapping with other poorly differentiated AL. We hypothesized that using standardized EuroFlow\u2122 Consortium approach could better diagnose such entities among cases that previously classified as acute myeloid leukemia (AML)\u2010M0, AML with minimal differentiation, AML with myelodysplasia\u2010related changes without further lineage differentiation, and AL of ambiguous lineage. In order to confirm this hypothesis and assess whether these AL subtypes such as BPDCN and ETP\u2010ALL had previously gone undetected, we reanalyzed 49 banked cryopreserved sample cases using standardized EuroFlow\u2122 Consortium panels. We also performed target sequencing to capture the mutational commonalities between these AL subtypes. Reanalysis led to revised or refined diagnoses for 23 cases (47%). Of these, five diagnoses were modified, uncovering 3 ETP\u2010ALL and 2 typical BPDCN cases. In 12 AML cases, a variable proportion of immature plasmacytoid dendritic cell and\/or monocytic component was newly identified. In one AML case, we have identified a megakaryoblastic differentiation. Finally, in five acute lymphoblastic leukemia (ALL) cases, we were able to more precisely determine the maturation stage. The application of standardized EuroFlow flow cytometry immunophenotyping improves the diagnostic accuracy of ALs and could impact treatment decisions.<\/jats:p>","DOI":"10.1002\/cyto.b.22213","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T04:45:01Z","timestamp":1731559501000},"page":"116-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Improved identification of clinically relevant <scp>Acute Leukemia<\/scp> subtypes using standardized <scp>EuroFlow<\/scp> panels versus non\u2010standardized approach"],"prefix":"10.1002","volume":"108","author":[{"given":"Rafik","family":"Terra","sequence":"first","affiliation":[{"name":"Division of Hematology, Oncology and Transplantation, Department of Medicine H\u00f4pital Maisonneuve\u2010Rosemont  Montr\u00e9al Quebec Canada"},{"name":"Faculty of Medicine Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Quebec 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Therapy University Institute, H\u00f4pital Maisonneuve\u2010Rosemont Research Center Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology BC Cancer  Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guylaine","family":"L\u00e9pine","sequence":"additional","affiliation":[{"name":"Division of Hematology, Oncology and Transplantation, Department of Medicine H\u00f4pital Maisonneuve\u2010Rosemont  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"LeBlanc","sequence":"additional","affiliation":[{"name":"Division of Hematology, Oncology and Transplantation, Department of Medicine H\u00f4pital 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