{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T05:03:01Z","timestamp":1748667781682,"version":"3.40.4"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T00:00:00Z","timestamp":1731888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The FDA\u2010approved ClearLLab 10C Reagents Panel (Beckman Coulter) simplified the diagnosis of leukemias and lymphomas by flow cytometry. However, the requirement of using 3 \u00d7\u200910<jats:sup>6<\/jats:sup> cells\/mL cannot be met for paucicellular samples. Therefore, we tested whether this 10\u2010color panel can be reliably employed to analyze specimens with low cell concentrations. Serial dilutions of 16 samples (5 normal, 11 abnormal), yielding concentrations ranging from 3.0 \u00d7\u200910<jats:sup>6<\/jats:sup> to 0.0469 \u00d7\u200910<jats:sup>6<\/jats:sup> cells\/mL (64\u2010fold difference), were stained using the B\u2010cell and T\u2010cell panels of the ClearLLab 10C system, and mean fluorescence intensity (MFI) was measured for each antibody. For each cell dilution, the deviation from the value obtained with the FDA\u2010approved concentration of 3.0 \u00d7\u200910<jats:sup>6<\/jats:sup> cells\/mL was calculated. The agreement between the highest and lowest cell concentration data was evaluated by the Bland and Altman method, Pearson's and Spearman's correlation analyses, and linear regression. In all patients, the antigen expression pattern was similar at all cell concentrations tested, and the mean deviation of the MFI from the value obtained using 3.0 \u00d7\u200910<jats:sup>6<\/jats:sup> cells\/mL never exceeded 10% for any of the antibodies. The Bland\u2013Altman method demonstrated the similarity between results obtained with the FDA\u2010approved cell concentration and a 64\u2010fold diluted cell suspension, and a high positive correlation was found between MFI acquired under these two conditions. The tests utilizing the lowest density of cells yielded the same patterns of antigen expression in all patients as those performed with the FDA\u2010approved concentration, documenting a 100% concordance between these two protocols. The ClearLLab 10C panel can reliably determine the expression of markers of leukemias and lymphomas in paucicellular samples containing as little as 0.0469 \u00d7\u200910<jats:sup>6<\/jats:sup> cells\/mL (64\u2010fold lower than the FDA\u2010approved concentration). This finding markedly expands the applicability of the ClearLLab 10C platform in a clinical setting.<\/jats:p>","DOI":"10.1002\/cyto.b.22215","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T05:19:59Z","timestamp":1731993599000},"page":"128-136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>ClearLLab 10C<\/scp> reagents panel can be applied to analyze paucicellular samples by flow cytometry"],"prefix":"10.1002","volume":"108","author":[{"given":"Ma\u0142gorzata","family":"Kajstura","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Rochester Medical Center  Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tia","family":"LaBarge","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Rochester Medical Center  Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew G.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Rochester Medical Center  Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V59.6.1292.1292"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02990721"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/096228029900800204"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jalm\/jfab176"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21981"},{"key":"e_1_2_6_9_1","first-page":"641","article-title":"Immune system reconstitution following allogeneic bone marrow transplantation in man: A multiparameter analysis","volume":"8","author":"Elfenbein G. 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Accessed May 5 2024."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2018.01.003"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.5858\/2006-130-680-CACFCI"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19981001)33:2<138::AID-CYTO8>3.0.CO;2-F"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T10:35:15Z","timestamp":1744540515000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,18]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/cyto.b.22215"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22215","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"type":"print","value":"1552-4949"},{"type":"electronic","value":"1552-4957"}],"subject":[],"published":{"date-parts":[[2024,11,18]]},"assertion":[{"value":"2024-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}