{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:23:34Z","timestamp":1792596214124,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007394","name":"Association Laurette Fugain","doi-asserted-by":"publisher","award":["ALF2017\u201015"],"award-info":[{"award-number":["ALF2017\u201015"]}],"id":[{"id":"10.13039\/100007394","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007394","name":"Association Laurette Fugain","doi-asserted-by":"publisher","award":["ALF2020\u201001"],"award-info":[{"award-number":["ALF2020\u201001"]}],"id":[{"id":"10.13039\/100007394","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201018\u2010IBHU\u20100003"],"award-info":[{"award-number":["ANR\u201018\u2010IBHU\u20100003"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la Recherche sur le Cancer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["INCa\u2010DGOS\u2010INSERM\u2010ITMO Cancer_18008"],"award-info":[{"award-number":["INCa\u2010DGOS\u2010INSERM\u2010ITMO Cancer_18008"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>BH3 profiling can assess global mitochondrial priming and dependence of leukemic cells on specific BH3 anti\u2010apoptotic proteins such as BCL\u20102. In acute myeloid leukemia (AML), proof\u2010of\u2010concept prognostic studies have been performed on archived samples variably accounting for molecular genetics. We undertook a single\u2010center feasibility study of a simplified flow\u2010based assay to determine the absolute mitochondrial priming and BCL\u20102 dependence in consecutive AML patients. When possible, results on the leukemic fraction were normalized to the cognate lymphocyte population (relative priming and BCL\u20102 dependence). Samples from 97 (89.8%) of the 108 referred patients were successfully processed. Relative priming and BCL\u20102 dependence could be determined in 62 (67.4%) and 67 (62.0%) samples, respectively. Absolute mitochondrial priming was lower in patients having previously failed intensive chemotherapy compared to chemotherapy\u2010na\u00efve patients (<jats:italic>p<\/jats:italic>\u2009=\u20090.01), but its prognostic impact was limited. Conversely, relative BCL\u20102 independence tended to predict worse EFS (HR\u2009=\u20092.51, <jats:italic>p<\/jats:italic>\u2009=\u20090.07) and OS (HR\u2009=\u20092.79, <jats:italic>p<\/jats:italic>\u2009=\u20090.10) independently of adverse genetic risk. Our results show that simplified BH3 profiling can be prospectively assessed in AML patients but that its prognostic use may require internal normalization. Future studies should compare its relevance with other functional assays such as ex vivo drug testing or BH3 protein expression.<\/jats:p>","DOI":"10.1002\/cyto.b.22217","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T08:38:24Z","timestamp":1732696704000},"page":"86-94","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prospective feasibility of a minimal <scp>BH3<\/scp> profiling assay in acute myeloid leukemia"],"prefix":"10.1002","volume":"108","author":[{"given":"Kim","family":"Pacchiardi","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, G\u00e9nomes, biologie cellulaire et th\u00e9rapeutique U944, INSERM, CNRS  Paris France"},{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pital Saint\u2010Louis, Assistance Publique\u2010H\u00f4pitaux de Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoire","family":"de Marcellus","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, G\u00e9nomes, biologie cellulaire et th\u00e9rapeutique U944, INSERM, CNRS  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Huynh","sequence":"additional","affiliation":[{"name":"D\u00e9partement H\u00e9matologie et Immunologie H\u00f4pital Saint\u2010Louis, Assistance Publique\u2010H\u00f4pitaux de Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofiane","family":"Fodil","sequence":"additional","affiliation":[{"name":"D\u00e9partement H\u00e9matologie et Immunologie H\u00f4pital Saint\u2010Louis, Assistance Publique\u2010H\u00f4pitaux de Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rathana","family":"Kim","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, G\u00e9nomes, biologie cellulaire et th\u00e9rapeutique U944, INSERM, CNRS  Paris 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