{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T09:55:30Z","timestamp":1780998930508,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>T lymphoblastic leukemia\/lymphoma (T\u2010ALL) is a malignancy composed of proliferating T lymphoblasts. T lymphoblasts can be identified by flow cytometric analysis through the detection of aberrant antigen expression and\/or immaturity marker expression. An ideal marker would be expressed brightly on T lymphoblasts but absent in mature T lymphocytes. One such marker is protein tyrosine kinase\u20107 (PTK7). However, PTK7 has not been widely adopted in clinical flow cytometry labs or incorporated into any T\u2010ALL flow cytometry best practice recommendations. To this end, we demonstrate the utility of PTK7 in flow cytometry panels for T\u2010ALL diagnosis, minimal\/measurable residual disease (MRD) detection, and relapse. We retrospectively evaluated flow cytometry data on 175 patients. PTK7 was classified as positive, showing a near two\u2010fold difference in brightness versus background mature T cells, in 87.76% of T\u2010ALL cases at initial diagnosis, 75% of T\u2010ALL at MRD, and 100% of T\u2010ALL at relapse. PTK7 expression remained intact in cases of CD34 and\/or TdT negative T\u2010ALL (<jats:italic>p<\/jats:italic>\u2009=\u20090.992) and while expression was dimmer at MRD (72% decrease, <jats:italic>p<\/jats:italic>\u2009=\u20090.0313), PTK7 remained intact at relapse (33% increase, <jats:italic>p<\/jats:italic>\u2009=\u20090.8125). PTK7 should be included in flow cytometry panels when evaluating for T\u2010ALL, both at initial diagnosis, relapse, and for the presence of MRD.<\/jats:p>","DOI":"10.1002\/cyto.b.22228","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T09:42:07Z","timestamp":1741686127000},"page":"320-328","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>PTK7<\/scp> helps detect T lymphoblastic leukemia\/lymphoma by flow cytometry"],"prefix":"10.1002","volume":"108","author":[{"given":"Jonah","family":"Maggard","sequence":"first","affiliation":[{"name":"University of Florida College of Medicine  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology Immunology and Laboratory Medicine  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Chaffin","sequence":"additional","affiliation":[{"name":"Department of Pathology Immunology and Laboratory Medicine  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Gars","sequence":"additional","affiliation":[{"name":"Department of Pathology Immunology and Laboratory Medicine  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology Immunology and Laboratory Medicine  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4404-8116","authenticated-orcid":false,"given":"Robert","family":"Seifert","sequence":"additional","affiliation":[{"name":"Department of Pathology Immunology and Laboratory Medicine  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01620-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015850"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21745"},{"key":"e_1_2_7_5_1","volume-title":"Validation of assays performed by flow cytometry","author":"CLSI","year":"2021"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-024-03060-x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21833"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-03-580027"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21518"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21866"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0039-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12068"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00031305.2018.1543616"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.htct.2020.09.148"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2012.07.004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12308-020-00406-8"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinepi.2016.02.031"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-338707"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21961"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-1766137"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2015.1070153"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/00031305.2019.1583913"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21108"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003656"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T04:40:30Z","timestamp":1753936830000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/cyto.b.22228"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22228","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]},"assertion":[{"value":"2024-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}