{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T19:37:12Z","timestamp":1754163432114,"version":"3.41.2"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Central nervous system (CNS) involvement in multiple myeloma (MM) is a rare but severe complication with a poor prognosis. The identification of malignant plasma cells in cerebrospinal fluid (CSF) is essential for early diagnosis and intervention. However, the sensitivity of traditional diagnostic methods like cytology is low, especially in samples with low\u2010cell counts. This study aimed to develop a multidimensional radar dot\u2010plot analysis using Kaluza software to enhance the sensitivity and specificity of flow cytometry for detecting abnormal plasma cells in CSF. One hundred and twenty\u2010five CSF samples were sent for flow cytometric testing to investigate the central nervous system involvement of MM. Finally, 89 samples from 40 patients were included in our study. Multicolor flow cytometry was performed using an 8\u2010color labeling method, and radar dot\u2010plot analysis was developed using diagnostic bone marrow samples to distinguish normal plasma cells, abnormal plasma cells, and cellular debris. The sensitivity of the novel method was evaluated by diluting myeloma bone marrow cells in pooled CSF samples to simulate low cell counts. Of the 125 CSF specimens, 16 samples from 4 patients showed abnormal plasma cells using both conventional and multidimensional flow cytometry analysis. Discordant results were found in 32 samples (25%), where conventional analysis suggested the presence of abnormal cells, but these were ruled out by multidimensional analysis. Sensitivity testing showed that the multidimensional dot\u2010plot method outperforms conventional two\u2010dimensional dot\u2010plot analysis, as the radar dot plot can be used to identify abnormal cells in samples diluted to 5\u2009WBC\/\u03bcL, where the cell count of abnormal plasma cells is &lt;\u20091\u2009cell\/\u03bcL. Our results showed that the new radar dot\u2010plot analysis can increase the sensitivity and specificity of flow cytometry in MM for the detection of CNS involvement, even in low\u2010cell\u2010count CSF samples, regardless of whether the sample was obtained in a tube containing special reagent or not (TransFix\/EDTA CSF Sample Storage tubes). This approach improves diagnostic accuracy, reduces the number of false positive cases caused by antibodies adhering to cell debris, and provides a reliable tool for assessing neurological complications in MM. Further validation is needed in a larger number of cases and testing of the method on different antibody panels.<\/jats:p>","DOI":"10.1002\/cyto.b.22245","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T08:09:18Z","timestamp":1751875758000},"page":"275-281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing detection of central nervous system involvement in multiple myeloma: A novel multidimensional dot\u2010plot based analysis for flow cytometry"],"prefix":"10.1002","volume":"108","author":[{"given":"Bettina","family":"Palicsk\u00f3","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine University of Debrecen  Debrecen Hungary"},{"name":"Laki K\u00e1lm\u00e1n Doctoral School, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Janov\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Rejt\u0151","sequence":"additional","affiliation":[{"name":"Department of Hematology Szabolcs\u2010Szatm\u00e1r\u2010Bereg County Teaching Hospital  Ny\u00edregyh\u00e1za Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"V\u00e1r\u00f3czy","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7364-9906","authenticated-orcid":false,"given":"Zsuzsanna","family":"Hevessy","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9489-8189","authenticated-orcid":false,"given":"Bettina","family":"K\u00e1rai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine University of Debrecen  Debrecen 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