{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:21:08Z","timestamp":1778692868536,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    To screen hereditary spherocytosis (HS), we first introduced the novel flow cytometric osmotic fragility test (FC\u2010OFT) based on the two\u2010point kinetic assay principle (FC\u2010OFT\n                    <jats:sub>Kinetic<\/jats:sub>\n                    ). With the introduction of FC systems with automatic tube loaders, we updated the FC\u2010OFT protocol to follow the endpoint assay principle (FC\u2010OFT\n                    <jats:sub>Endpoint<\/jats:sub>\n                    ). This study aims to evaluate the updated FC\u2010OFT protocol (FC\u2010OFT\n                    <jats:sub>Endpoint<\/jats:sub>\n                    ) and compare its assay performance with that of FC\u2010OFT\n                    <jats:sub>Kinetic<\/jats:sub>\n                    . We investigated factors influencing the FC\u2010OFT\n                    <jats:sub>Endpoint<\/jats:sub>\n                    assay, optimized its protocol, and defined the cutoff index using 152 negative or artificially positive control samples. We then compared the assay performance with that of FC\u2010OFT\n                    <jats:sub>Kinetic<\/jats:sub>\n                    in 25 patients with anemia, including 14 with spherocytosis\u20148, 4, 1, and 1 with HS, autoimmune hemolytic anemia, burn injury, and liver cirrhosis, respectively. To optimize FC\u2010OFT\n                    <jats:sub>Endpoint<\/jats:sub>\n                    , we adopted phosphate\u2010buffered saline as the red cell suspension medium, 50% deionized water for hypotonic osmotic pressure in adults, and a 3\u2010min standby time. This FC\u2010OFT\n                    <jats:sub>Endpoint<\/jats:sub>\n                    was more accurate than FC\u2010OFT\n                    <jats:sub>Kinetic<\/jats:sub>\n                    in identifying spherocytosis in the 25 patients with anemia (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0313). FC\u2010OFT\n                    <jats:sub>Endpoint<\/jats:sub>\n                    is a viable alternative to conventional OFT or FC\u2010OFT\n                    <jats:sub>Kinetic<\/jats:sub>\n                    for HS screening in clinical laboratories, as automatic FC enhances assay performance. These findings warrant validation in future multicenter studies with larger sample sizes.\n                  <\/jats:p>","DOI":"10.1002\/cyto.b.22254","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T13:11:28Z","timestamp":1760447488000},"page":"456-465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of flow cytometric osmotic fragility test between kinetic and endpoint assay principle"],"prefix":"10.1002","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1983-2515","authenticated-orcid":false,"given":"Dong Il","family":"Won","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology School of Medicine, Kyungpook National University  Daegu Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eui Yeol","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Kyungpook National University Hospital  Daegu Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Mook","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Kyungpook National University Hospital  Daegu Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye Jee","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Pediatrics School of Medicine, Kyungpook National University  Daegu Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12794"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05052.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21511"},{"key":"e_1_2_7_5_1","volume-title":"StatPearls","author":"Kaufman D. P.","year":"2025"},{"key":"e_1_2_7_6_1","first-page":"427","article-title":"The effect of EDTA, heparin and storage on the erythrocyte osmotic fragility, plasma osmolality and haematocrit of adult ostriches (Struthio camelus)","volume":"77","author":"Mafuvadze B.","year":"2007","journal-title":"Veterinarski Arhives"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2013.02.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21733"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPO7V4OGXLIIPP"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-5994.1976.tb03282.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22206"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22032"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21143"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V46.6.955.955"},{"key":"e_1_2_7_15_1","first-page":"205","article-title":"Acquired spherocytosis associated with thermal injury","volume":"6","author":"Thompson R. B.","year":"1965","journal-title":"Journal of the Mississippi State Medical Association"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.294.8.924"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20583"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20448"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/2211068214532254"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.22254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T08:51:09Z","timestamp":1764406269000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.22254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,14]]},"references-count":18,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/cyto.b.22254"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.22254","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,14]]},"assertion":[{"value":"2025-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}