{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:25:56Z","timestamp":1791476756950,"version":"4.3.4"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100025530","name":"Else Kr\u00f6ner Fresenius Center for Digital Health","doi-asserted-by":"publisher","award":["Digital Clinician Trialist Program 2026"],"award-info":[{"award-number":["Digital Clinician Trialist Program 2026"]}],"id":[{"id":"10.13039\/501100025530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Measurable residual disease (MRD) monitoring by multiparameter flow cytometry (MFC) is well established in bone marrow (BM) samples for acute myeloid leukemia (AML), but its use in peripheral blood (PB) remains less developed. We adapted a semi\u2010automated and well validated MFC\u2010MRD assay, originally developed for BM, to PB aiming to evaluate its applicability and concordance with established methods. To transfer the MFC\u2010MRD assay from BM to PB, we incorporated population\u2010specific reference values derived from healthy and chemotherapy\u2010exposed non\u2010AML controls. MRD detection was based on a combined aberrant leukemia associated immunophenotype (aLAIP) and different from normal (DfN) approach. We compared MFC\u2010MRD detection in paired PB and BM samples and evaluated concordance with molecular MRD (Mol\u2010MRD) diagnostics. Additional analyses included assessment of mast cells as markers for BM hemodilution as well as time requirements for analyses. Reference values for aberrant populations in PB were generally lower than in BM, particularly considering immature markers. At follow\u2010up, MFC\u2010MRD positivity was less common in PB than in BM, leading to a concordance rate of 73.7%. Both quantitative and qualitative differences in aberrant antigen expression between PB and BM were observed. Most cases of MRD positivity were characterized by newly emerging aberrant features rather than persistent ones. Concordance between MFC\u2010MRD from PB and Mol\u2010MRD from BM was 64.6%, similar to that of MFC\u2010MRD from BM versus Mol\u2010MRD from BM (58.5%). Most discordant cases (MFC\u2010MRD\n                    <jats:sup>pos<\/jats:sup>\n                    \/Mol\u2010MRD\n                    <jats:sup>neg<\/jats:sup>\n                    ) may reflect clonal hematopoiesis or limited spectrum of molecular panels. Mast cell quantification proved useful in identifying hemodiluted BM samples, which comprised ~15% of cases. Inter\u2010rater agreement for MRD detection was strong (\n                    <jats:italic>K\u03b1<\/jats:italic>\n                    \u2009&gt;\u20090.8). The MFC\u2010MRD gating and evaluation was rapid (~2\u2009min\/sample in both PB and BM). The adapted MFC\u2010MRD assay is feasible and robust in PB, offering a fast and reproducible alternative to BM\u2010based MRD monitoring. However, the prognostic relevance of the proposed method needs to be validated in a prospective cohort of patients.\n                  <\/jats:p>","DOI":"10.1002\/cyto.b.70019","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T12:55:07Z","timestamp":1771246507000},"page":"259-265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Measurable residual disease by multicolor flow cytometry in acute myeloid leukemia: A comparison of peripheral blood and bone marrow"],"prefix":"10.1002","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2687-9178","authenticated-orcid":false,"given":"Jonas","family":"Schadt","sequence":"first","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"},{"name":"Else Kr\u00f6ner Fresenius Center for Digital Health, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malte","family":"von Bonin","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2287-437X","authenticated-orcid":false,"given":"Uta","family":"Oelschl\u00e4gel","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-0548","authenticated-orcid":false,"given":"Christoph","family":"R\u00f6llig","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Thiede","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"},{"name":"AgenDix GmbH  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo","family":"Ruhnke","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5916-3029","authenticated-orcid":false,"given":"Martin","family":"Bornh\u00e4user","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"},{"name":"National Center of Tumor Diseases  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Brenner","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8213-2047","authenticated-orcid":false,"given":"Maximilian Alexander","family":"R\u00f6hnert","sequence":"additional","affiliation":[{"name":"Department of Medicine I Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster D. 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Reviews"},{"key":"e_1_2_8_3_1","unstructured":"Brando B. &Gatti A.(2019).Rules in rare event acquisition\u2014An overview.Presented at the European Society for Clinical Cell Analysis 2019 Bergen (Norway).https:\/\/www.escca.eu\/images\/publication\/PAR-08_Brando_ESCCA_Rare_Event_Analysis_2019_revised.pdf"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022016867"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.29"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020006219"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0393-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17348"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021013626"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.19446"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2958.2004.tb00738.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/20406207221115005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10432"},{"key":"e_1_2_8_14_1","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2024"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01647-5"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-024-02158-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20008"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.255"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.70019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.70019","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.70019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:37:23Z","timestamp":1785854243000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.70019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,16]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/cyto.b.70019"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.70019","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,16]]},"assertion":[{"value":"2025-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}