{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:59:15Z","timestamp":1785481155967,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Assessment of T\u2010cell clonality is critical for diagnosing T\u2010cell non\u2010Hodgkin lymphomas (T\u2010NHL). Flow cytometric T\u2010cell receptor (TCR)\u2010V\u03b2\u2010repertoire analysis is specific but technically complex, multi\u2010tube, and often limited in paucicellular specimens. T\u2010cell receptor\u2010beta\u2010constant\u2010region\u20101 (TRBC1) has emerged as a simpler alternative, but normal distribution ranges in clinically relevant T\u2010cell subsets remain insufficiently characterized and data on direct comparison with TCR\u2010V\u03b2 restriction is limited. We prospectively analyzed 70 blood samples from healthy donors and 58 samples (bone marrow, peripheral blood, fine\u2010needle aspirates, body fluids) from T\u2010NHL patients. Multiparameter flow cytometry (MFC) was used for diagnostic immunophenotyping in T\u2010NHL cases and for TRBC1 assessment in T\u2010NHL cases and 28 immunophenotypic T\u2010cell subsets in healthy individuals. In T\u2010NHL, clonality was determined by TRBC1 restriction in aberrant T\u2010cell populations and, where sufficient material was available, corroborated by TCR\u2010V\u03b2 repertoire analysis (IOTest Beta Mark).In healthy donors, median TRBC1\u2010positive fractions across T\u2010cell subsets ranged from 21.39% to 78.61%, with narrow 99% confidence intervals in major CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    compartments. Minor subsets occasionally showed extreme values (7%\u201393%) but remained polyclonal by TCR\u2010V\u03b2 analysis, leading to thresholds of \u22646% or \u226594% TRBC1 positivity as indicative of clonality in our cohort. Among 57 evaluable T\u2010NHL cases, all CD3\u2010positive neoplastic populations showed monotypic TRBC1 expression, with 100% concordance with TCR\u2010V\u03b2 results in 18 tested cases. TRBC1 was non\u2010informative on surface\u2010CD3\u2010negative T\u2010NHL, although cytoplasmic staining was sometimes helpful. Flow cytometric TRBC1 analysis is a rapid, cost\u2010effective, single\u2010tube method for T\u2010cell clonality assessment, with applicability to small T\u2010cell subsets such as paucicellular and measurable residual disease samples.\n                  <\/jats:p>","DOI":"10.1002\/cyto.b.70054","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:23:54Z","timestamp":1785479034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detailed analysis of\n                    <scp>TRBC1<\/scp>\n                    distribution in T\u2010cell subsets and its application in T\u2010cell clonality assessment"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0284-603X","authenticated-orcid":false,"given":"Anumeha","family":"Chaturvedi","sequence":"first","affiliation":[{"name":"Hematopathology and Cytogenetics Section, Department of Pathology, Homi Bhabha Cancer 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