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We collected an extensive dataset containing 5366 flow cytometry files from 885 AML patients, stained with the EuroFlow acute leukemia orientation tube (ALOT) and AML\/MDS panel, acquired in a standardized way at eight centers over a period of 10\u2009years. Unsupervised clustering identified groups of patients based on FlowSOM\u2010derived cell population percentages. In addition, we investigated immunophenotypic patterns in World Health Organization (WHO) patient classes and\n                    <jats:italic>NPM1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>mut<\/jats:italic>\n                    <\/jats:sup>\n                    subclasses, both at the cell population and the individual marker level. Some WHO classes, for example, AML with t(8;21)(q22;q22)\/\n                    <jats:italic>RUNX1::RUNX1T1<\/jats:italic>\n                    or t(15;17)(q24;q21)\/\n                    <jats:italic>PML::RARA<\/jats:italic>\n                    , showed homogeneous immunophenotypes. Characterization of maturation arrest using FlowSOM confirmed maturation arrest at early stages in distinct WHO classes. Finally, a machine learning model was trained to predict WHO genetic classes from immunophenotypic data. The model allowed accurate prediction in 77% of cases, reproducible in an independent validation cohort. In conclusion, we show that EuroFlow standardized protocols allow analysis of multi\u2010centric data measured over an extended period of time. Computational analysis demonstrated inter\u2010 and intrapatient immunophenotypic heterogeneity and allowed prediction of genetic abnormalities.\n                  <\/jats:p>","DOI":"10.1002\/cyto.b.70077","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:10:08Z","timestamp":1790687408000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Old data, new tricks: Comprehensive computational analysis of 10 years of multi\u2010center\n                    <scp>EuroFlow<\/scp>\n                    acute myeloid leukemia diagnostic immunophenotypic data"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0637-4893","authenticated-orcid":false,"given":"Sarah","family":"Bonte","sequence":"first","affiliation":[{"name":"Data Mining and Modeling for Biomedicine VIB\u2010UGent Center for Inflammation Research  Ghent Belgium"},{"name":"Department of Mathematics, Computer Science and Statistics Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosan","family":"Olsman","sequence":"additional","affiliation":[{"name":"Laboratory Medical Immunology, Department of Immunology Erasmus MC, University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Van Gassen","sequence":"additional","affiliation":[{"name":"Data Mining and Modeling for Biomedicine VIB\u2010UGent Center for Inflammation Research  Ghent Belgium"},{"name":"Department of Mathematics, Computer Science and Statistics Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Matarraz","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Program, Centro de Investigaci\u00f3n del C\u00e1ncer (IBMCC; CSIC\u2010University of Salamanca); Cytometry Service, NUCLEUS; Department of Medicine University of Salamanca (USAL) and Institute of Biomedical Research of Salamanca (IBSAL)  Salamanca Spain"},{"name":"Biomedical Research Networking Centre Consortium of Oncology (CIBERONC) Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neus","family":"Villamor","sequence":"additional","affiliation":[{"name":"Unitat d'Hematopatologia, Hospital C\u00ednic and Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS) Centro de Investigaci\u00f3n Biom\u00e9dica en Red, c\u00e1ncer (CIBERONC)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Nierkens","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht The Netherlands"},{"name":"Center for Translational Immunology, UMC Utrecht  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Labormedizin Kantonsspital Aarau AG  Aarau Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-5816","authenticated-orcid":false,"given":"Elaine Sobral","family":"da Costa","sequence":"additional","affiliation":[{"name":"Pediatrics Department, Faculty of Medicine Universidade Federal do Rio de Janeiro (UFRJ)  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen\u2010Mariana","family":"Aanei","sequence":"additional","affiliation":[{"name":"Inserm U1059, Sainbiose, Facult\u00e9 de M\u00e9decine Jacques Lisfranc Universit\u00e9 de Lyon  Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Program, Centro de Investigaci\u00f3n del C\u00e1ncer (IBMCC; CSIC\u2010University of Salamanca); Cytometry Service, NUCLEUS; Department of Medicine University of Salamanca (USAL) and Institute of Biomedical Research of Salamanca (IBSAL)  Salamanca Spain"},{"name":"Biomedical Research Networking Centre Consortium of Oncology (CIBERONC) Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J. M.","family":"van Dongen","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Program, Centro de Investigaci\u00f3n del C\u00e1ncer (IBMCC; CSIC\u2010University of Salamanca); Cytometry Service, NUCLEUS; Department of Medicine University of Salamanca (USAL) and Institute of Biomedical Research of Salamanca (IBSAL)  Salamanca Spain"},{"name":"Biomedical Research Networking Centre Consortium of Oncology (CIBERONC) Instituto de Salud Carlos III  Madrid Spain"},{"name":"Department of Immunology LUMC  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvan","family":"Saeys","sequence":"additional","affiliation":[{"name":"Data Mining and Modeling for Biomedicine VIB\u2010UGent Center for Inflammation Research  Ghent Belgium"},{"name":"Department of Mathematics, Computer Science and Statistics Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Hofmans","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Sciences Ghent University  Ghent Belgium"},{"name":"Department of Laboratory Medicine Ghent University Hospital  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9457-3763","authenticated-orcid":false,"given":"Vincent H. 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