{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T23:21:09Z","timestamp":1773876069939,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012505","name":"Royal Perth Hospital Medical Research Foundation","doi-asserted-by":"publisher","award":["RIIG 274\/2024"],"award-info":[{"award-number":["RIIG 274\/2024"]}],"id":[{"id":"10.13039\/100012505","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Myelofibrosis is a myeloproliferative neoplasm with potential to transform to acute myeloid leukemia. This evolution is unpredictable and current assays lack the sensitivity and applicability needed to predict this transformation. While population\u2010level data utilizing comprehensive genomic profiling can identify subgroups at higher risk of progression, they do not provide individualized information on the likelihood or timing of leukemia. Cytogenetic alterations are typically present in secondary leukemia. We aimed to determine whether these changes could be detected at an early stage. To achieve this we established and tested a single\u2010cell imaging flow cytometric method for chromosomal aberrations using fluorescence in\u00a0situ hybridization (FISH) probes to analyze circulating CD34\/CD45\u2010positive cells. Peripheral blood samples from 14 patients, collected at up to eight timepoints over a 34\u2010month period, were analyzed for defects involving chromosomes 1, 5, and 17. Following cell immunophenotyping and FISH probe hybridization, a mean of 174,216 mononuclear cells was assessed per sample. Chromosomal abnormalities including gain(1q), del(5q), idic (5), monosomy 17, and\/or del(17p) were identified in eight patients, at frequencies down to 0.2% of mononuclear cells. Serial analyses revealed emergence of new chromosomal lesions, clonal evolution, dominance, and multi\u2010hit abnormalities. In three patients, acquired chromosome 17 abnormalities preceded progression to secondary leukemia by up to 7\u2009months. This pilot study demonstrates that imaging flow cytometry\u2010based FISH of circulating CD34\/CD45\u2010positive cells enables real\u2010time, blood\u2010based surveillance for cytogenetic evolution in myelofibrosis. The ability to dynamically track clone size and hierarchy highlights its potential as an early predictor of leukemic transformation in myelofibrosis.<\/jats:p>","DOI":"10.1002\/cytoa.70012","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T07:35:47Z","timestamp":1770190547000},"page":"98-107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Imaging Flow Cytometry Detection of Cytogenetic Abnormalities in Circulating\n                    <scp>CD34<\/scp>\n                    + Cells Predicts Leukemic Transformation in Myelofibrosis"],"prefix":"10.1002","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8152-4182","authenticated-orcid":false,"given":"Ruby M.","family":"Hamilton","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4973-4502","authenticated-orcid":false,"given":"Ryan J.","family":"Collinson","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0442-6987","authenticated-orcid":false,"given":"Henry Y.","family":"Hui","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi Yun","family":"Ng","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"},{"name":"Department of Haematology Royal Perth Hospital  Perth WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2722-9018","authenticated-orcid":false,"given":"Hun S.","family":"Chuah","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"},{"name":"Department of Haematology Royal Perth Hospital  Perth WA Australia"},{"name":"PathWest Laboratory Medicine  Nedlands WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm","family":"Webb","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"},{"name":"Hollywood Hospital Hollywood  Nedlands WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2146-8561","authenticated-orcid":false,"given":"Belinda B.","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1028-9376","authenticated-orcid":false,"given":"Wendy N.","family":"Erber","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"},{"name":"Department of Haematology Royal Perth Hospital  Perth WA Australia"},{"name":"PathWest Laboratory Medicine  Nedlands WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-7501","authenticated-orcid":false,"given":"Kathy A.","family":"Fuller","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Western Australia  Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1406186"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12071810"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/hs9.0000000000000333"},{"key":"e_1_2_9_5_1","volume-title":"WHO Classification of Haematolymphoid Tumours","author":"WHO Classification of Tumours Editorial Board","year":"2024"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10123551"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11010253"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277\u2010022\u201004862\u20103"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010023\u201000878\u20108"},{"issue":"10","key":"e_1_2_9_10_1","first-page":"1123","article-title":"Clinical Utility of the Absolute Number of Circulating CD34\u2010Positive Cells in Patients With Chronic Myeloproliferative Disorders","volume":"88","author":"Passamonti F.","year":"2003","journal-title":"Haematologica"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1716614"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2020.100708"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020002271"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010018\u20100107\u2010z"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018024018"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010018\u20100018\u2010z"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102011\u201001\u2010331678"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2010.32.2446"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2018.78.9867"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2017.11.003"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.260"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14248"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102011\u201008\u2010372904"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25230"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23769"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2005.04.010"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201007\u2010297598"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2010.04.006"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cytoa.70012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cytoa.70012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cytoa.70012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T15:42:56Z","timestamp":1773848576000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cytoa.70012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/cytoa.70012"],"URL":"https:\/\/doi.org\/10.1002\/cytoa.70012","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-11-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}