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We examined seven AD blood biomarkers (amyloid beta [, , , total tau [t\u2010tau], neurofilament light chain [NfL],  and ) in three collection tube types (ethylenediaminetetraacetic acid [EDTA] plasma, heparin plasma, serum).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma and serum were obtained from cerebrospinal fluid or amyloid positron emission tomography\u2010positive and \u2010negative participants (N\u00a0=\u00a038) in the Wisconsin Registry for Alzheimer's Prevention. We modeled AD biomarker values observed in EDTA plasma versus heparin plasma and serum, and assessed correspondence with brain amyloidosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results suggested bias due to tube type, but crosswalks are possible for some analytes, with excellent model fit for NfL (=\u00a00.94), adequate for amyloid (=\u00a00.40\u20100.69), and weaker for t\u2010tau (=\u00a00.04\u20100.42) and  ( =\u00a00.22\u20100.29). Brain amyloidosis differentiated several measures, especially EDTA plasma  (=\u00a01.29).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>AD biomarker concentrations vary by tube type. However, correlations for some biomarkers support harmonization across types, suggesting cautious optimism for use in banked blood.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dad2.12266","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:30:36Z","timestamp":1644453036000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Crosswalk study on blood collection\u2010tube types for Alzheimer's disease biomarkers"],"prefix":"10.1002","volume":"14","author":[{"given":"Erin M.","family":"Jonaitis","sequence":"first","affiliation":[{"name":"School of Medicine and Public Health Wisconsin Alzheimer's Institute University of Wisconsin\u2013Madison  Madison Wisconsin USA"},{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Zetterberg","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology University of Gothenburg  M\u00f6lndal Sweden"},{"name":"Clinical Neurochemistry Laboratory Institute of Neuroscience and Physiology Sahlgrenska University Hospital  M\u00f6lndal Sweden"},{"name":"Department of Neurodegenerative Disease UCL Institute of Neurology  London UK"},{"name":"UK Dementia Research Institute at UCL  London UK"},{"name":"Hong Kong Center for Neurodegenerative Diseases  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca Langhough","family":"Koscik","sequence":"additional","affiliation":[{"name":"School of Medicine and Public Health Wisconsin Alzheimer's Institute University of Wisconsin\u2013Madison  Madison Wisconsin USA"},{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobey J.","family":"Betthauser","sequence":"additional","affiliation":[{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol A.","family":"Van Hulle","sequence":"additional","affiliation":[{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Hegge","sequence":"additional","affiliation":[{"name":"School of Medicine and Public Health Wisconsin Alzheimer's Institute University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendlyn","family":"Kollmorgen","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH  Penzberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivonne","family":"Suridjan","sequence":"additional","affiliation":[{"name":"Roche Diagnostics International Ltd  Rotkreuz Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carey E.","family":"Gleason","sequence":"additional","affiliation":[{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"},{"name":"Geriatric Research Education and Clinical Center of the Wm. 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Middleton Memorial Veterans Hospital  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne D.","family":"Engelman","sequence":"additional","affiliation":[{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"},{"name":"Department of Population Health Sciences University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozioma C.","family":"Okonkwo","sequence":"additional","affiliation":[{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Asthana","sequence":"additional","affiliation":[{"name":"Wisconsin Alzheimer's Disease Research Center School of Medicine and Public Health University of Wisconsin\u2013Madison  Madison Wisconsin USA"},{"name":"Geriatric Research Education and Clinical Center of the Wm. 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