{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T22:06:48Z","timestamp":1773266808990,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alz &amp; Dem Diag Ass &amp; Dis Mo"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>INTRODUCTION<\/jats:title><jats:p>Our previous antibody\u2010based cerebrospinal fluid (CSF) proteomics study showed that Thimet oligopeptidase (THOP1), an amyloid beta (A\u03b2) neuropeptidase, was increased in mild cognitive impairment with amyloid pathology (MCI\u2010A\u03b2+) and Alzheimer's disease (AD) dementia compared with controls and dementia with Lewy bodies (DLB), highlighting the potential of CSF THOP1 as an early specific biomarker for AD. We aimed to develop THOP1 immunoassays for large\u2010scale analysis and validate our proteomics findings in two independent cohorts.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>We developed in\u2010house CSF THOP1 immunoassays on automated Ella and Simoa platforms. The performance of the different assays were compared using Passing\u2013Bablok regression analysis in a subset of CSF samples from the discovery cohort (<jats:italic>n<\/jats:italic> = 72). Clinical validation was performed in two independent cohorts (cohort 1: <jats:italic>n<\/jats:italic> = 200; cohort 2: <jats:italic>n<\/jats:italic> = 165) using the Ella platform.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>THOP1 concentrations moderately correlated between proteomics analysis and our novel assays (<jats:italic>Rho<\/jats:italic>\u00a0&gt;\u00a00.580). In both validation cohorts, CSF THOP1 was increased in MCI\u2010A\u03b2+ (&gt;1.3\u2010fold) and AD (&gt;1.2\u2010fold) compared with controls; and between MCI\u2010A\u03b2+ and DLB (&gt;1.2\u2010fold). Higher THOP1 concentrations were detected in AD compared with DLB only when both cohorts were analyzed together. In both cohorts, THOP1 correlated with CSF total tau (t\u2010tau), phosphorylated tau (p\u2010tau), and A\u03b240 (<jats:italic>Rho<\/jats:italic>\u00a0&gt;\u00a00.540) but not A\u03b242.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>Validation of our proteomics findings underpins the potential of CSF THOP1 as an early specific biomarker associated with AD pathology. The use of antibody\u2010based platforms in both the discovery and validation phases facilitated the translation of proteomics findings, providing an additional workflow that may accelerate the development of biofluid\u2010based biomarkers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dad2.12456","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T05:39:55Z","timestamp":1690349995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Thimet oligopeptidase as a potential CSF biomarker for Alzheimer's disease: A cross\u2010platform validation study"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9713-116X","authenticated-orcid":false,"given":"Yanaika S.","family":"Hok\u2010A\u2010Hin","sequence":"first","affiliation":[{"name":"Neurochemistry Laboratory, Department of Laboratory Medicine, Amsterdam Neuroscience VU University Medical Center, Amsterdam UMC 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