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However, the diagnosis of dementia still relies primarily on cerebrospinal fluid and imaging modalities. The new disease\u2010modifying treatments call for more widely applicable biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Plasma samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a0250) from two mixed memory clinic were included. Participants were divided into amyloid beta positives (A\u03b2+) and A\u03b2 negatives (A\u03b2\u2212). Plasma phosphorylated tau (p\u2010tau) 181, p\u2010tau231, A\u03b21\u201042 (A\u03b242), A\u03b240, A\u03b242\/A\u03b240, glial fibrillary acidic protein (GFAP), and neurofilament light chain (NfL) were measured by single molecule array.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Significant differences were found among cognitively unimpaired, mild cognitive impairment, Alzheimer's disease (AD), and non\u2010AD, and nearly all of the biomarkers were able to predict amyloid status. When combining p\u2010tau181 and p\u2010tau231 they predicted A\u03b2 positivity with an area under the curve (AUC) of 0.75, and when combining all biomarkers an AUC of 0.86 was found.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>This study supports previous findings on plasma biomarkers, even when investigated in a typical clinical setting in a consecutive, heterogeneous, mixed memory clinic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>This study investigated seven plasma biomarkers in a mixed memory clinic, regardless of amyloid co\u2010pathology or atypical phenotypes.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings support previous promising results on plasma biomarkers, even when investigated in a heterogeneous population.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The combination of phosphorylated tau (p\u2010tau)181 and p\u2010231 performed only slightly worse than a panel of multiple biomarkers, aligning with previous studies.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Plasma biomarkers show potential for future applications in primary care, treatment monitoring, and trial selection.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/dad2.70073","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T07:05:45Z","timestamp":1737011145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Plasma biomarkers of amyloid, tau, astrogliosis, and axonal injury in a mixed memory clinic cohort"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7470-4818","authenticated-orcid":false,"given":"Helena Sophia","family":"Gleerup","sequence":"first","affiliation":[{"name":"Department of Neurology Danish Dementia Research Centre Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja Hviid","family":"Simonsen","sequence":"additional","affiliation":[{"name":"Department of Neurology Danish Dementia Research Centre Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lana","family":"Gr\u00f6tschel","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neurochemistry Institute of Neuroscience &amp; Physiology the Sahlgrenska Academy at the University of Gothenburg  M\u00f6lndal Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias Holsey","family":"Gramkow","sequence":"additional","affiliation":[{"name":"Department of Neurology Danish Dementia Research Centre Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"H\u00f8gh","sequence":"additional","affiliation":[{"name":"Regional Dementia Research Centre, Department of Neurology Zealand University Hospital  Roskilde Denmark"},{"name":"Department of Clinical Medicine Faculty of Health and Medical Science University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaj","family":"Blennow","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neurochemistry Institute of Neuroscience &amp; Physiology the Sahlgrenska Academy at the University of Gothenburg  M\u00f6lndal Sweden"},{"name":"Clinical Neurochemistry Laboratory Sahlgrenska University Hospital  M\u00f6lndal Sweden"},{"name":"Paris Brain Institute, ICM Piti\u00e9\u2010Salp\u00eatri\u00e8re Hospital Sorbonne University, H\u00f4pital Piti\u00e9  Paris France"},{"name":"Neurodegenerative Disorder Research Center Division of Life Sciences and Medicine and Department of Neurology Institute on Aging and Brain Disorders University of Science and Technology of China and First Affiliated Hospital of USTC  Hefei P.R. 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