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Foghts Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009908","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alz &amp;amp; Dem Diag Ass &amp;amp; Dis Mo"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>Differentiating dementia with Lewy bodies (DLB) from Alzheimer's disease (AD) is challenging. Seed amplification assay (SAA) is sensitive for the detection of misfolded \u03b1\u2010synuclein.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      Patients with DLB (\n                      <jats:italic>N<\/jats:italic>\n                      \u00a0=\u00a031) and AD (\n                      <jats:italic>N<\/jats:italic>\n                      \u00a0=\u00a025) were recruited and evaluated. Misfolded \u03b1\u2010synuclein was assessed in cerebrospinal fluid (CSF), skin, urine, and olfactory mucosa using SAA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The accuracy of \u03b1\u2010synuclein\u2010SAA for DLB was 87% (95% confidence interval [CI]: 77% to 98%) in CSF, 85% (95% CI: 75% to 98%) in skin, 58% (95% CI: 47% to 69%) in olfactory mucosa, and 59% (95% CI: 51% to 66%) in urine. The core symptoms \u2013 fluctuations, REM sleep behavior disorder, and parkinsonism \u2013 had accuracies for SAA positivity of\u00a0\u226579%. Notably, 95% of SAA\u2010positive patients also had hyposmia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>These findings support the use of CSF and skin \u03b1\u2010synuclein\u2010SAAs as diagnostic tools for DLB, with strong associations between SAA and clinical phenotype. In particular, intact olfactory function is associated with a lower risk of SAA positivity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>CSF and skin biopsies show high diagnostic accuracy for \u03b1\u2010synuclein, demonstrating good concordance.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Strong correlations exist between core symptoms of DLB and pathological \u03b1\u2010synuclein.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>A very high sensitivity of hyposmia for pathological \u03b1\u2010synuclein is observed.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>A novel proof\u2010of\u2010concept is offered for the potential detection of pathological \u03b1\u2010synuclein in urine, marking the first such comparative analysis between patients with DLB and AD.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dad2.70203","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:03:43Z","timestamp":1762517023000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["\u03b1\u2010Synuclein seed amplification assay in Lewy body dementia versus Alzheimer's disease"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9728-8773","authenticated-orcid":false,"given":"Oskar H.","family":"McWilliam","sequence":"first","affiliation":[{"name":"Danish Dementia Research Centre Department of Neurology Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remarh","family":"Bsoul","sequence":"additional","affiliation":[{"name":"Department of Pathology Danish Reference Center for Prion Diseases Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva L.","family":"Lund","sequence":"additional","affiliation":[{"name":"Department of Pathology Danish Reference Center for Prion Diseases Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"},{"name":"Department of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunhild","family":"Waldemar","sequence":"additional","affiliation":[{"name":"Danish Dementia Research Centre Department of Neurology Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"},{"name":"Department of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steen G.","family":"Hasselbalch","sequence":"additional","affiliation":[{"name":"Danish Dementia Research Centre Department of Neurology Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"},{"name":"Department of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja H.","family":"Simonsen","sequence":"additional","affiliation":[{"name":"Danish Dementia Research Centre Department of Neurology Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Bruun","sequence":"additional","affiliation":[{"name":"Danish Dementia Research Centre Department of Neurology Copenhagen University Hospital \u2010 Rigshospitalet  Copenhagen Denmark"},{"name":"Department of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"von Buchwald","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Otolaryngology Head &amp; 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