{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:00:05Z","timestamp":1785582005223,"version":"3.56.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":75,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alz &amp;amp; Dem Diag Ass &amp;amp; Dis Mo"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>\n                      Altered copper metabolism has been reported in Alzheimer's disease (AD), with\n                      <jats:italic>post mortem<\/jats:italic>\n                      brain tissue showing depletion of\n                      <jats:sup>65<\/jats:sup>\n                      Cu compared with\n                      <jats:sup>63<\/jats:sup>\n                      Cu. Whether such isotopic shifts can be detected in biofluids remains unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>We analyzed copper isotope composition in human cerebrospinal fluid (CSF) and serum from AD patients and controls using multi\u2010collector inductively coupled plasma mass spectrometry (MC\u2010ICP\u2010MS) and single quadrupole inductively coupled plasma mass spectrometry (SQ\u2010ICP\u2010MS). Samples included 22 CSFs (10 AD, 12 controls), 54 sera (28 AD, 26 controls).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      AD CSF was enriched in\n                      <jats:sup>65<\/jats:sup>\n                      Cu (+0.32\u2030,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.02), correlating inversely with CSF amyloid beta (A\u03b2)\n                      <jats:sub>42\/40<\/jats:sub>\n                      ratios. Serum from AD patients was depleted in\n                      <jats:sup>65<\/jats:sup>\n                      Cu (\u22120.23\u2030,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.013), trending inversely with amyloid positron emission tomography burden. Serum and CSF\n                      <jats:italic>\u03b4<\/jats:italic>\n                      <jats:sup>65<\/jats:sup>\n                      Cu diagnostically discriminate between AD and controls (area under the curve\u00a0=\u00a00.8, Youden's J \u2248 0.5 SD,\n                      <jats:italic>p<\/jats:italic>\n                      \u2264 0.0002).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>Copper isotope excursions in brain parenchyma are mirrored in CSF and serum. These shifts reflect amyloid pathology and highlight isotopic copper analysis as a potential minimally invasive biomarker for AD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dad2.70316","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T02:57:13Z","timestamp":1781578633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Copper isotope shifts in biofluids track Alzheimer's pathology"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3009-3235","authenticated-orcid":false,"given":"Esther","family":"Lahoud","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Institut de Physique du Globe de Paris, CNRS  Paris 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