{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:40:57Z","timestamp":1792554057773,"version":"4.5.0"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alz &amp; Dem Diag Ass &amp; Dis Mo"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>Blood\u2010based biomarkers may improve access to Alzheimer's disease diagnosis, but use may be limited in clinics without on\u2010site processing capability. We evaluated the stability of the plasma phosphorylated tau 217 (pTau217)\/amyloid beta 42 (A\u03b242) ratio after delayed whole\u2010blood processing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>We compared blood processed within 2\u00a0h or held at 4\u00b0C for approximately 24 or 48\u00a0h before centrifugation and analysis using the Fujirebio Lumipulse G1200. Stability was assessed using ordinary least squares regression, error index, Bland\u2010Altman analysis, and Deming regression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Correlations with the 2\u2010h condition were high at both 24 and 48\u00a0h (\n                      <jats:italic>R<\/jats:italic>\n                      \u00a0=\u00a00.998). Both delayed conditions met prespecified regression and error\u2010index criteria. Mean absolute percent error was 5.6% at 24\u00a0h and 9.8% at 48\u00a0h. Deming regression showed no constant bias, although proportional bias was observed at 48\u00a0h. No bias was observed for individual analytes for up to 48\u00a0h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>The pTau217\/A\u03b242 ratio in refrigerated whole blood remained stable for 24\u00a0h, with possible utility up to 48\u00a0h, supporting more flexible specimen\u2010transport workflows.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dad2.70517","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:07:36Z","timestamp":1792552056000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stability of plasma pTau217\/A\u03b242 ratio with delayed blood processing"],"prefix":"10.1002","volume":"18","author":[{"given":"Hana","family":"Mayfield","sequence":"first","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casey S.","family":"John","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenae","family":"Pennington","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riley E.","family":"Kemna","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maggie","family":"Kroeger","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amitava","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"University of Kansas Health System Department of Pathology and Laboratory Medicine  Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Burns","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny M.","family":"Elahi","sequence":"additional","affiliation":[{"name":"Friedman Brain Institute Ronald M. Loeb Center for Alzheimer's Disease Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melody","family":"Nelson","sequence":"additional","affiliation":[{"name":"University of Kansas Health System Department of Pathology and Laboratory Medicine  Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill K.","family":"Morris","sequence":"additional","affiliation":[{"name":"University of Kansas Medical Center Department of Neurology  Kansas City Kansas USA"},{"name":"University of Kansas Alzheimer's Disease Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlaa014"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.121.263195"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.05.017"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awag001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13859"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/trc2.70013"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlaf115"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.70752"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15757"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178943"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014\u2010017\u20109174\u20109"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014\u2010016\u20109126\u20109"}],"container-title":["Alzheimer's &amp; Dementia: Diagnosis, Assessment &amp; Disease Monitoring"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dad2.70517","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dad2.70517","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dad2.70517","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:07:38Z","timestamp":1792552058000},"score":1,"resource":{"primary":{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/10.1002\/dad2.70517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/dad2.70517"],"URL":"https:\/\/doi.org\/10.1002\/dad2.70517","archive":["Portico"],"relation":{},"ISSN":["2352-8729","2352-8729"],"issn-type":[{"value":"2352-8729","type":"print"},{"value":"2352-8729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70517"}}