{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T13:46:15Z","timestamp":1785678375923,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,10,5]],"date-time":"2004-10-05T00:00:00Z","timestamp":1096934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The distinction of cystic squamous\u2010cell carcinoma (SCC) from benign cystic squamous lesions (BCSLs) of the head and neck can be problematic on fine\u2010needle aspiration biopsy (FNAB) material, particularly when BCSLs display epithelial reactive atypia or when SCC is well differentiated. Glucose transporter 1 (GLUT\u20101), a facilitative cell surface glucose transport protein, is aberrantly expressed in many cancers including oral and hypopharyngeal SCC. We evaluated the expression of GLUT\u20101 by immunochemistry on FNAB material to determine its value in distinguishing cystic SCC from BCSL of the head and neck.<\/jats:p><jats:p>A 5\u2010yr retrospective review of all head and neck cystic squamous lesions having FNAB specimens with cell block material, radiological studies, and histological confirmation was performed at our institution. Cell block material from 24 cystic squamous lesions, including 8 (33%) BCSL (7 branchial cleft cysts and 1 thyroglossal duct cyst[TDC]) and 16 (67%) metastatic SCCs with cystic\/liquefactive degeneration, was retrieved and immunostained with anti\u2010GLUT\u20101. GLUT\u20101 expression was considered positive when at least 10% of squamous cells exhibited distinct cell membrane reactivity.<\/jats:p><jats:p>Positive GLUT\u20101 immunostaining was detected in all 16 SCCs and in none of the 8 BCSLs. In the carcinoma cases, the majority of malignant cells exhibited GLUT\u20101 reactivity; only a minor population of well\u2010differentiated SCC cells displaying keratinization and arranged as squamous pearls did not express GLUT\u20101.<\/jats:p><jats:p>GLUT\u20101 expression in cell block material can help to distinguish cystic SCCs from BCSLs of the head and neck. In conjunction with clinical and radiological correlation, GLUT\u20101 immunoreactivity can be an important diagnostic aid when the cytological findings are ambiguous. Diagn. Cytopathol. 2004;31:294\u2013299. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.20127","type":"journal-article","created":{"date-parts":[[2004,10,5]],"date-time":"2004-10-05T23:01:49Z","timestamp":1097017309000},"page":"294-299","source":"Crossref","is-referenced-by-count":21,"title":["Diagnostic value of GLUT\u20101 immunoreactivity to distinguish benign from malignant cystic squamous lesions of the head and neck in fine\u2010needle aspiration biopsy material"],"prefix":"10.1002","volume":"31","author":[{"given":"Michael F.","family":"Weiner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto N.","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo H.","family":"Bardales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perkins","family":"Mukunyadzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra J.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheila","family":"Korourian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis E.","family":"De Las Casas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(89)90242-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/019459988108900113"},{"key":"e_1_2_1_4_2","first-page":"201","article-title":"Inflamed branchial cyst: a potential pitfall in aspiration cytology","volume":"30","author":"Warson F","year":"1986","journal-title":"Acta Cytol"},{"key":"e_1_2_1_5_2","first-page":"494","article-title":"Metastatic squamous cell carcinoma presenting as a neck cyst. 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