{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:28:36Z","timestamp":1791379716574,"version":"4.3.3"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,1,29]],"date-time":"2008-01-29T00:00:00Z","timestamp":1201564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>p16<jats:sup>INK4a<\/jats:sup>, cyclin\u2010dependent kinase inhibitor, is functionally inactivated in many tumors, including cervical cancer. We compared p16<jats:sup>INK4A<\/jats:sup> immunocytochemical staining and Hybrid Capture\u00ae 2 (HCII) on SurePath\u2122 specimens using tissue biopsies (as the gold standard). Their utility in a spectrum of atypical and preneoplastic lesions, and their ability to accurately identify underlying lesions of CIN II or greater was assessed using biopsy follow\u2010up data. One\u2010hundred and seventeen residual SurePath\u2122 samples were collected: 43 atypical squamous cells of undetermined significance (ASCUS), 47 low\u2010grade (LGSIL), and 27 high\u2010grade (HGSIL) squamous intraepithelial lesions. Two slides were prepared from each sample; one stained with the SurePath\u2122 autocyte stain and one immunostained using the CINtec\u2122 p16<jats:sup>INK4a<\/jats:sup> Cytology Kit (Dakocytomation). High\u2010risk HPV testing was performed using the HCII DNA test (Digene, Gaithersburg, MD). Available tissue biopsy follow\u2010up data was retrieved. p16<jats:sup>INK4a<\/jats:sup> was positive in 32.6% (14\/43) ASCUS, 46.8% (22\/47) LGSIL, and 48.1% (13\/27) HGSIL specimens. HCII DNA test was positive in 41.9% (18\/43) ASCUS, 78.7% (37\/47) LGSIL, and 96.3% (26\/27) HGSIL samples. The sensitivity, specificity, positive (PPV) and negative (NPV) predictive values of p16<jats:sup>INK4a<\/jats:sup> and HCII were: 58.7% and 89.8%, 58.6% and 34.6%, 69.2% and 72.1%, 47.2% and 64.3%, respectively. In patients with cervical biopsies, the PPV of HCII (92.3%) results for a biopsy with CINII\/III was significantly higher than the PPV of p16<jats:sup>INK4a<\/jats:sup> (52%) (<jats:italic>P<\/jats:italic> = 0.001). Using liquid\u2010based cytology specimens, HCII is a more sensitive test than p16<jats:sup>INK4a<\/jats:sup> for detection of abnormal cytology. HCII has a higher PPV than p16<jats:sup>INK4a<\/jats:sup> for identifying CIN II\/III. Diagn. Cytopathol. 2008;36:142\u2013148. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.20770","type":"journal-article","created":{"date-parts":[[2008,1,29]],"date-time":"2008-01-29T20:48:24Z","timestamp":1201639704000},"page":"142-148","source":"Crossref","is-referenced-by-count":9,"title":["Comparison of p16<sup>INK4A<\/sup> and Hybrid Capture\u00ae 2 human papillomavirus testing as adjunctive tests in liquid\u2010based gynecologic SurePath\u2122 preparations"],"prefix":"10.1002","volume":"36","author":[{"given":"Aziza","family":"Nassar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"O'Reilly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momin 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