{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T19:58:09Z","timestamp":1700596689158},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,4,16]],"date-time":"2008-04-16T00:00:00Z","timestamp":1208304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our objective was to determine the morphotype of the adherent bacteria in liquid\u2010based cytology (LBC) in smears with healthy and disturbed vaginal flora. And to use PCR technology on the same fixed cell sample to establish DNA patterns of the 16S RNA genes of the bacteria in the sample. Thirty samples were randomly selected from a large group of cervical cell samples suspended in a commercial coagulant fixative \u201c(BoonFix).\u201d PCR was used to amplify DNA of five bacterial species: <jats:italic>Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus jensenii, Gardnerella vaginalis<\/jats:italic>, and <jats:italic>Mycoplasma hominis<\/jats:italic>. The LBC slides were then analyzed by light microscopy to estimate bacterial adhesion. DNA of lactobacilli was detected in all cell samples. Seventeen smears showed colonization with <jats:italic>Gardnerella vaginalis<\/jats:italic> (range 2.6 \u00d7 10<jats:sup>2<\/jats:sup>\u20133.0 \u00d7 10<jats:sup>5<\/jats:sup> bacteria\/\u03bcl BoonFix sample). Two cases were identified as dysbacteriotic with high DNA values for <jats:italic>Gardnerella vaginalis<\/jats:italic> and low values for <jats:italic>Lactobacillus crispatus<\/jats:italic>. The sample with the highest concentration for <jats:italic>Gardnerella vaginalis<\/jats:italic> showed an unequivocal <jats:italic>Gardnerella<\/jats:italic> infection. This study indicates that the adherence pattern of a disturbed flora in liquid\u2010based cervical samples can be identified unequivocally, and that these samples are suitable for quantitative PCR analysis. This cultivation independent method reveals a strong inverse relationship between <jats:italic>Gardnerella vaginalis<\/jats:italic> and <jats:italic>Lactobacillus crispatus<\/jats:italic> in dysbacteriosis and unequivocal <jats:italic>Gardnerella<\/jats:italic> infection. Diagn. Cytopathol. 2008;36:277\u2013284. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.20793","type":"journal-article","created":{"date-parts":[[2008,4,16]],"date-time":"2008-04-16T19:16:54Z","timestamp":1208373414000},"page":"277-284","source":"Crossref","is-referenced-by-count":6,"title":["<i>Gardnerella vaginalis<\/i> and <i>Lactobacillus<\/i> sp in liquid\u2010based cervical samples in healthy and disturbed vaginal flora using cultivation\u2010independent methods"],"prefix":"10.1002","volume":"36","author":[{"given":"Johanna M.","family":"Klomp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Banut\u2010Sabine M.","family":"Verbruggen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Korporaal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathilde E.","family":"Boon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pauline","family":"de Jong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerco C.","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maarten","family":"van Haaften","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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