{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:54:12Z","timestamp":1792166052938,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One of the major handicaps in contemporary clinical oncology is the inability to predict the responsiveness of any individual's malignancy to specific therapies. The purpose of this study was to test the feasibility of immunocytochemically detecting markers that may be affected by therapy or are predictive of therapeutic responsiveness, including phosphohistone H1 (anti\u2010p\u2010H1 MoAb 12D11) and X\u2010linked inhibitor of apoptosis (XIAP) in small samples obtained via fine\u2010needle aspiration (FNA) biopsy procedure, thus improving therapeutic monitoring. p63, a squamous stem cell regulatory protein, was also examined. These three markers were studied in FNA cell block samples of head and neck squamous cell carcinoma (HNSCC). Twenty\u2010eight alcohol\u2010fixed formalin\u2010postfixed paraffin\u2010embedded cell\u2010block samples from FNAs of patients with HNSCC were subjected to antigen retrieval and then incubated with anti\u2010XIAP, anti\u2010p\u2010H1, and anti\u2010p63, and developed using EnVision\u2010Plus reagents and diaminobenzidine as chromagen; Granular or heterogeneous cytoplasmic staining for XIAP and nuclear staining for p63 and p\u2010H1 were considered positive. Among the 28 cases studied, the overall positive rates for XIAP, p\u2010H1, and p63 were 60.7%, 96.4%, and 92.8%, respectively. The staining intensity for XIAP: + 70.6%, ++ 23.5%, +++ 0%, and ++++ 5.9%; for p\u2010H1: + 48.1%, ++ 11.1%, +++37.0%, and ++++ 3.7%; and for p63: + 11.5%, ++ 23.1%, +++ 53.9%, and ++++ 11.5%. The expression of p\u2010H1 and p63 appeared to be higher and stronger than that of XIAP in HNSCC. This study demonstrated the feasibility of monitoring expression of three tumor markers using FNA samples. p\u2010H1 and XIAP may be useful for monitoring actions of cyclin\u2010dependent kinase inhibitors, XIAP\u2010lowering, and\/or apoptosis\u2010inducing drugs, respectively. Future studies will focus on the impact of therapies upon these staining profiles. Diagn. Cytopathol. 2008;36:797\u2013800. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.20919","type":"journal-article","created":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T17:11:44Z","timestamp":1222881104000},"page":"797-800","source":"Crossref","is-referenced-by-count":10,"title":["Feasibility of immunocytochemical detection of tumor markers (XIAP, phosphohistone H1 and p63) in FNA cellblock samples from head and neck squamous cell carcinoma"],"prefix":"10.1002","volume":"36","author":[{"given":"M.","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kafanas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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