{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:42:12Z","timestamp":1760298132408},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,9,16]],"date-time":"2009-09-16T00:00:00Z","timestamp":1253059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Even though p16<jats:sup>INK4a<\/jats:sup> (p16) immunocytochemistry has proven a useful accessory tool verifying the identification of atypical squamous cells of undetermined significance (ASC\u2010US) categorized smears, the procedure still has limitations. To date few studies examining the usefulness of p16 immunocytochemistry in atypical squamous cells which cannot exclude HSIL (ASC\u2010H), compared with ASC\u2010US in liquid\u2010based cervical smears. Therefore, we examined the correlation of p16 immunocytochemical staining with follow\u2010up biopsy results on ASC\u2010H categorized smears and compared the data with those classified as ASC\u2010US on 105 liquid\u2010based cytology samples. We found no statistical significance in the p16 expression of ASC\u2010US smears and the presence of squamous intraepithelial lesions (SIL) in follow\u2010up biopsies (p = 0.546). However, p16 expression did significantly correlate with the presence of SIL (p = 0.002) in ASC\u2010H smears. There was a statistically significant relationship between p16 expression and presence of high grade squamous intraepithelial lesions (HSIL) or more on the follow\u2010up biopsies in both ASC\u2010US (p = 0.012) and ASC\u2010H (p &lt; 0.001) categorized smears. In ASC\u2010US categorized smears, there was no statistical significance between p16 expression and the HR\u2010HPV viral load (p = 0.091). But there was a statistical significance between p16 expression and the HR\u2010HPV viral load (p &lt; 0.001) in ASC\u2010H categorized smears. Our results indicate that p16 immunostaining is a much better useful marker for HR\u2010HPV infection and detection of SIL in ASC\u2010H categorized smears compared to those defined as ASC\u2010US. Diagn. Cytopathol. 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.21164","type":"journal-article","created":{"date-parts":[[2009,9,16]],"date-time":"2009-09-16T18:59:10Z","timestamp":1253127550000},"page":"168-171","source":"Crossref","is-referenced-by-count":14,"title":["The use of p16<sup>INK4A<\/sup> immunocytochemistry in \u201cAtypical squamous cells which cannot exclude HSIL\u201d compared with \u201cAtypical squamous cells of undetermined significance\u201d in liquid\u2010based cervical smears"],"prefix":"10.1002","volume":"38","author":[{"given":"Chang Ohk","family":"Sung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Rim","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Lyun","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Yong","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,9,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_1_3_2","first-page":"391","article-title":"The Papanicolaou smear: Its value and limitations","volume":"42","author":"Lieu D","year":"1996","journal-title":"J Fam Pract"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-8258(02)00102-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.18.2382"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.38698.458866.7C"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djj035"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.4.293"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800551"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.RCT.0000157092.44680.25"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20175"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000325839"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2004.11.034"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20786"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11589"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.01.043"},{"key":"e_1_2_1_17_2","first-page":"584","article-title":"Aberrant methylation during cervical carcinogenesis","volume":"7","author":"Virmani AK","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200606000-00003"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.21164","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.21164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T23:46:45Z","timestamp":1700610405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.21164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,16]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1002\/dc.21164"],"URL":"https:\/\/doi.org\/10.1002\/dc.21164","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,16]]}}}