{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T23:38:33Z","timestamp":1772667513579,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T00:00:00Z","timestamp":1259020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>ProEx\u2122 C and p16<jats:sup>INK4a<\/jats:sup> staining of cytology\/histology specimens have recently been explored to help distinguish high\u2010grade squamous intraepithelial lesions (HSIL) from benign mimics. The goal of this study was to evaluate the performance characteristics of p16 and ProEx C in tissue and patient matched ThinPrep\u00ae liquid\u2010based cytology specimens. Residual cervical ThinPrep cytology specimens and tissue blocks (<jats:italic>N<\/jats:italic> = 64) from 63 patients were stained with p16 and ProEx C. Review of immunostained material, Papanicolaou and H&amp;E stained slides was performed by two cytopathologists. The cytology slides were evaluated for the presence or absence of squamous atypia as well as immunoreactivity. Histologic specimens were interpreted as negative, indeterminate, or positive for each immunostain. There was 86% agreement (55\/64) between the p16 and ProEx C stains on tissue specimens. Eleven specimens were interpreted as positive for both stains. All had a low\u2010 or high\u2010grade squamous lesion on the corresponding H&amp;E section. ProEx C was able to identify four low\u2010grade squamous intraepithelial lesion specimens that were interpreted as negative by p16. All four HSIL specimens demonstrated p16 and ProEx C staining. However, 84% of cytology negative specimens demonstrated false\u2010positive staining. Clinical utilization of both stains, combined with morphologic features, may be beneficial for confirming HSIL on histologic specimens. ProEx C and\/or p16 immunostains may lead to a false\u2010positive result in cytology specimens due to staining of normal appearing cells. Diagn. Cytopathol. 2010;38:564\u2013572. 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.21251","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T15:45:48Z","timestamp":1259077548000},"page":"564-572","source":"Crossref","is-referenced-by-count":16,"title":["Comparison of p16<sup>INK4a<\/sup> and ProEx\u2122 C immunostaining on cervical ThinPrep\u00ae cytology and biopsy specimens"],"prefix":"10.1002","volume":"38","author":[{"given":"Trynda N.","family":"Oberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin R.","family":"Kipp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie A.","family":"Vrana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary 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