{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:58:33Z","timestamp":1758632313984},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T00:00:00Z","timestamp":1273795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The main aim of this work is to present a special method for conjunctival impression cytology (CIC) and objective measures of measure cell and nucleus dimensions.<\/jats:p><jats:p>CIC using Millicell\u00ae Biopore filters was obtained from 50 Caucasian adults from the nasal bulbar conjunctiva. Giemsa\u2010stained images of cells in a monolayer were photographed with a scale bar and projected at 1,500\u00d7 to generate an overlay. Measures were made of both the long (L) and short (S) dimensions of the cells and the longest dimension of the nucleus (LN). The selected images comprised sets of 10 from different individuals, which had been assigned grades 0, 1, 2, or 3 and an additional grade 0.5.<\/jats:p><jats:p>L ranged from 18.4 to 65.2 \u03bcm, S from 13.1 to 47.1 \u03bcm, to give average cell shape values (L:S ratio) of between 1.259 and 1.890. L and S were smallest for grade 0 cells (group mean 19.8 \u00b1 1.1 and 14.8 \u00b1 1.2 \u03bcm) and largest for grade 3 cells (group 51.2 \u00b1 7.2 and 37.2 \u00b1 4.7 \u03bcm), with no difference in L:S ratios across the cell grades. Longest dimension of the nucleus (NL) values ranged from 8.7 to 14.5 \u03bcm, with group mean values of 9.8 \u00b1 0.6 \u03bcm for grade 0 cells and 12.9 \u00b1 1.2 \u03bcm for grade 3 cells. NL was nonlinearly associated with L.<\/jats:p><jats:p>Measures of conjunctival cell and nucleus dimensions can be used to objectively distinguish between cells assigned different grades of squamous metaplasia, with slight nucleus enlargement being the earliest detectable change (at grade 0.5). Diagn. Cytopathol. 2011;39:409\u2013423. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.21404","type":"journal-article","created":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T19:59:16Z","timestamp":1273867156000},"page":"409-423","source":"Crossref","is-referenced-by-count":20,"title":["Objective assessment of conjunctival squamous metaplasia by measures of cell and nucleus dimensions"],"prefix":"10.1002","volume":"39","author":[{"given":"Michael J.","family":"Doughty","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,5,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7506-4196-8.50004-3"},{"key":"e_1_2_6_3_2","first-page":"485","article-title":"New insights into the surface cells and glands of the conjunctiva and their relevance to the tear film","volume":"74","author":"Doughty MJ","year":"2003","journal-title":"Optometry"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1444-0938.2004.tb03098.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1475-1313.2002.00013.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70301-4"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(77)90499-8"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.1997.tb00569.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.85.2.154"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.1997.tb00567.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010225)93:1<60::AID-CNCR9008>3.0.CO;2-5"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(85)33967-2"},{"key":"e_1_2_6_13_2","first-page":"431","article-title":"Estudio ultrastuctural de la conjunctiva en pacientes con queratoconjunctivitis seca","volume":"70","author":"Alvarez MI","year":"1996","journal-title":"Arch Soc Esp Oftalmol"},{"key":"e_1_2_6_14_2","first-page":"245","article-title":"Tratamientos con eledoisina en pacientes con queratoconjunctivitis seca","volume":"70","author":"Alvarez MI","year":"1996","journal-title":"Arch Soc Esp Oftalmol"},{"key":"e_1_2_6_15_2","first-page":"437","article-title":"Citolog\u00eda de impressi\u00f3n aplicada al diagn\u00f3stico de la conjunctivitis","volume":"71","author":"\u00c1lvarez MI","year":"1996","journal-title":"Arch Soc Esp Oftalomol"},{"key":"e_1_2_6_16_2","first-page":"206","article-title":"Conjnuctival morphology in Sjogrens syndrome and other disorders of the anterior eye. 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