{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:23:44Z","timestamp":1790987024136,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,7,6]],"date-time":"2010-07-06T00:00:00Z","timestamp":1278374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>p16 immunostaining has been examined to detect high\u2010grade cervical intraepithelial neoplasia grade (CIN2+) in Pap cytology specimens. However, the utility of p16 in predicting CIN2+ in Pap specimens with atypical squamous cells of undetermined significance (ASC\u2010US) or atypical squamous cells, cannot exclude high\u2010grade squamous intraepithelial neoplasm (ASC\u2010H), is controversial. In this study, we evaluated the utility of p16 immunostaining for predicting CIN2+ in 78 Pap specimens with ASC\u2010US\/ASC\u2010H and compared the results in high\u2010risk HPV DNA and the follow\u2010up biopsies. p16 immunostaining was positive in 47% (37\/78) of the Pap specimens. Of the 13 Pap specimens with follow\u2010up biopsy results of CIN2+, 7 (54%) were positive for p16. p16 positivity in the Pap specimens was not significantly associated with a CIN2+ biopsy result (P = 0.76). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of p16 immunostaining for predicting CIN2+ were 54%, 52%, 19%, and 85%, respectively. High\u2010risk HPV DNA was detected in 40% (31\/78) of the Pap specimens. The sensitivity, specificity, PPV, and NPV of HPV DNA for predicting CIN2+ were 100%, 72%, 42%, and 100%, respectively. High\u2010risk HPV genotypes were detected in six p16\u2010negative specimens with follow\u2010up biopsy results of CIN2+. Our findings suggest that the utility of p16 immunostaining for predicting CIN2+ in Pap specimens with ASC\u2010US\/ASC\u2010H is limited. Scant abnormal cells in Pap specimens with ASC\u2010US\/ASC\u2010H may have contributed to the low p16 sensitivity. Diagn. Cytopathol., 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.21415","type":"journal-article","created":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T03:35:06Z","timestamp":1278473706000},"page":"482-488","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of p16 immunostaining to predict high\u2010grade cervical intraepithelial neoplasia in women with Pap results of atypical squamous cells of undetermined 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