{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T10:44:14Z","timestamp":1756464254925},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,8]],"date-time":"2010-12-08T00:00:00Z","timestamp":1291766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fine\u2010needle aspiration (FNA) biopsy (FNAB) in the preoperative assessment of breast lesions has shown diagnostic limitations with false\u2010positive and false\u2010negative diagnoses. We investigated the diagnostic value of cytokeratin 5\/6 (CK5\/6) and p63 in a series of breast FNABs, diagnosed as proliferative breast lesions with or without atypia, to see whether these ancillary studies enhance the ability to make an accurate diagnosis by FNAB. Sixty\u2010four breast FNABs were retrieved between January 2000 and December 2005 and included in the study as follows: 29\/64 (45%) cases as proliferative with atypia and 35\/64 (55%) without atypia. We also included 10 cases of fibroadenoma. All cases had histological follow\u2010up available for correlation. Immunostaining for CK5\/6 and p63 was performed on the cell block material in all cases. The percentage of staining cells in the specimen was graded as 0 (0\u201310%), 1 (11\u201325%), 2 (26\u201350%), and 3 (&gt;50%). There were 9\/29 (31%) cases in the atypical group that were found to be malignant on resection, compared with 6\/35 (17%) in the cases without atypia. In histologically proven malignant cases, CK5\/6 was negative in 11\/15 (73%) or showed 1+ stain in 2\/15 (13%) cases. In benign breast lesions, CK5\/6 stained more than 25% of cell proliferation in 44\/49 (90%). p63 showed characteristic staining for single naked bipolar nuclei in the background of the specimen (not appreciated by CK5\/6) in all fibroadenoma cases. In conclusion, CK5\/6 may enhance the ability to differentiate between benign and malignant epithelial proliferations in breast FNABs. In fibroepithelial lesions, p63 may be more useful than CK5\/6. Diagn. Cytopathol. 2011. \u00a9 2010 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.21534","type":"journal-article","created":{"date-parts":[[2010,12,8]],"date-time":"2010-12-08T14:37:24Z","timestamp":1291819044000},"page":"141-147","source":"Crossref","is-referenced-by-count":8,"title":["The diagnostic utility of CK5\/6 and p63 in fine\u2010needle aspiration of the breast lesions diagnosed as proliferative fibrocystic lesion"],"prefix":"10.1002","volume":"40","author":[{"given":"Hatem","family":"Al\u2010Maghraby","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeina","family":"Ghorab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walid","family":"Khalbuss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan F.","family":"Silverman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reda S.","family":"Saad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,8]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"898","article-title":"The spectrum of the \u201cgray zone\u201d in breast cytology. 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