{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:09:04Z","timestamp":1757704144896},"reference-count":7,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,10,29]],"date-time":"2001-10-29T00:00:00Z","timestamp":1004313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report on the formulation and use of an alcoholic\u2010agar additive solution generally useful for rapid, inexpensive liquid\u2010based cytology slide preparation. Gynecological cytology specimens were collected from 1,000 women. Two hundred fifty aliquots each of CytoRich, CytoRich Red (Tripath Imaging, Inc., Burlington, NC), Preservcyt (Cytyc Corp., Boxborough, MA), and DINA*TRANS (DINA*CYT Corp., Portland, OR) fixatives were used for this study. Fixed cell suspensions from 1,000 women, seen consecutively by a general obstetrics and gynecology practice, were vortex\u2010mixed and transferred into a premeasured amount of alcoholic\u2010agar in test tubes. Test tubes were conventionally centrifuged, cells were trapped in a spontaneously formed agar\u2010gel, and the supernatant solutions were decanted. Vortex\u2010mixing cell buttons caused a rapid gel\u2010to\u2010sol transition, affording viscous cell suspensions that were applied to slides, smeared, and stained using Papanicolaou stains. Slides showed unclumped, monolayered, uniform, random cell\u2010spreads. All of the fixatives afforded crisp presentation of normal and abnormal cells. There was an about 3\u2010fold increase in HSIL+ (0.7\u20131.8%) and LSIL diagnoses (1.3\u20134.4%), and a 45% reduction in ASCUS diagnoses (3.3\u20131.8%), as compared to our cytology laboratory's previous year's patients' statistics with a concurrent 0.2% unsatisfactory rate, due solely to inadequate sampling. This manual method makes liquid\u2010based cytology inexpensive and does not require specialized preparative devices. Diagn. Cytopathol. 25:334\u2013338, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dc.2166","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:15:54Z","timestamp":1030310154000},"page":"334-338","source":"Crossref","is-referenced-by-count":22,"title":["Manual method for liquid\u2010based cytology: A demonstration using 1,000 gynecological cytologies collected directly to vial and prepared by a smear\u2010slide technique"],"prefix":"10.1002","volume":"25","author":[{"given":"John A.","family":"Maksem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Finnemore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beverly L.","family":"Belsheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen B.","family":"Roose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukanya R.","family":"Makkapati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorie","family":"Eatwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Weidmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_2","series-title":"Code of Federal Regulations title 21","first-page":"209","author":"United States Food and Drug Administration","year":"2000"},{"key":"e_1_2_1_3_2","first-page":"1","article-title":"Should labs buy the new cytotechnology: a simple cost analysis","volume":"3","author":"IOMA","year":"2000","journal-title":"IOMA Med Lab Manage Rep"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(97)00228-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000331545"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.4.293"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0339(200102)24:2<137::AID-DC1027>3.0.CO;2-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000330957"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.2166","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.2166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T16:02:27Z","timestamp":1700409747000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.2166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,29]]},"references-count":7,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["10.1002\/dc.2166"],"URL":"https:\/\/doi.org\/10.1002\/dc.2166","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,29]]}}}