{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T08:23:52Z","timestamp":1758875032098},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T00:00:00Z","timestamp":1307404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Most guidelines currently recommend the testing of human papillomavirus (HPV) in ASCUS cases. The most used method for this purpose is Hybrid Capture II (HCII), but PCR techniques with GP5+\/6+ primers can be also applied. Furthermore, the HCII high\u2010risk probe test for detection of HPV shows cross\u2010reactivity with low\u2010risk HPV. Although this cross\u2010reactivity has been studied in screening populations, it has received little attention in ASCUS cases.<\/jats:p><jats:p>To compare the performance of the HCII high\u2010risk probe test and PCR for the detection of HPV in ASCUS cases.<\/jats:p><jats:p>We randomly selected 83 ASCUS cases that were positive for high\u2010risk HPV by HCII and applied the PCR test using MYO9\u201011 and GP5+\/6+ primers to samples from these cases.<\/jats:p><jats:p>Our results show cross\u2010reactivity with low\u2010risk HPV in 25.3% (21\/83) of the HCII+ PCR+ cases. Regarding the follow\u2010up our results emphasize the importance of HPV typing, especially for HPV 16 infection.<\/jats:p><jats:p>We propose the use of PCR techniques using GP5+\/6+ consensus primers for the screening of HPV in ASCUS. Diagn. Cytopathol. 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.21724","type":"journal-article","created":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T21:40:01Z","timestamp":1307482801000},"page":"1043-1046","source":"Crossref","is-referenced-by-count":4,"title":["PCR study of a series of ASCUS cases HPV\u2010positive by HCII"],"prefix":"10.1002","volume":"40","author":[{"given":"Francesc","family":"Alameda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Bellosillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"Lloveras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Pairet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merc\u00e8","family":"Musset","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Pijuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lluisa","family":"Mari\u00f1oso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gemma","family":"Mancebo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Larrazabal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n","family":"Carreras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergi","family":"Serrano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.03.077"},{"key":"e_1_2_6_3_2","first-page":"1394","article-title":"Restricted cross\u2010reactivity of hybrid capture 2 with nononcogenic human papillomavirus types","volume":"11","author":"Castle PE","year":"2002","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22952"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"3248","DOI":"10.1128\/JCM.36.11.3248-3254.1998","article-title":"Comparison of PCR\u2010 and HC\u2010based HPV detection systems using multiple cervical specimen collection strategies","volume":"36","author":"Peyton CV","year":"1998","journal-title":"J Clin 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